抗 2,4-二氯苯酚和 2,4-二氯苯氧乙酸单克隆抗体的制备。
Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies.
机构信息
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higahshi-ku, Fukuoka, 812-8582, Japan.
出版信息
Cytotechnology. 2003 Jul;42(2):101-7. doi: 10.1023/B:CYTO.0000009919.62901.27.
The ratios of hapten and bovine serum albumin (BSA) in an antigen conjugate were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Hybridomas secreting monoclonal antibodies against 2,4-dichlorophenoxyacetic acid (2,4-D) were produced by fusing 2,4-D-BSA conjugate-immunized splenocytes with a HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A substantial cross-reaction was observed for 2,4-dichlorophenol (2,4-DP) when compared with that observed for 2,4-D. The full measurement range for this assay is 0.2-3 mug ml(-1) for 2,4-DP. On the other hand, the range for 2,4-D is between 1 and 20 mug ml(-1).
采用基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱法测定半抗原与牛血清白蛋白（BSA）在抗原缀合物中的比例。通过将 2,4-二氯苯氧乙酸（2,4-D）-BSA 缀合物免疫的脾细胞与 HAT 敏感的小鼠骨髓瘤细胞系 P3-X63-Ag8-653 融合，产生了针对 2,4-D 的单克隆抗体杂交瘤。与 2,4-D 相比，观察到 2,4-二氯苯酚（2,4-DP）有明显的交叉反应。该测定法的全测量范围为 2,4-DP 的 0.2-3μgml（-1）。另一方面，2,4-D 的范围在 1 至 20μgml（-1）之间。
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