Chapel F, Fabiani B, Davi F, Raphael M, Tepper M, Champault G, Guettier C
Service d'Anatomie et de Cytologie Pathologiques, Hôpital Jean Verdier, Bondy, France.
Histopathology. 2000 Mar;36(3):252-61. doi: 10.1046/j.1365-2559.2000.00843.x.
The presence of Epstein-Barr virus (EBV) was studied in 56 gastric carcinomas from Western patients by in-situ hybridization for EBV-encoded RNAs (EBER). EBV-positive and negative carcinomas were compared for various pathological parameters including p53 overexpression.
EBERs transcripts were detected in seven cases overall: four cases of 52 conventional carcinomas (7. 7%) and three cases of four gastric carcinomas with lymphoid stroma (75%). EBER positivity was diffuse in five cases and restricted to a localized area of the tumour in two cases of conventional carcinoma. A monoclonal EBV genomic pattern was demonstrated in the case tested by Southern blot analysis. By immunohistochemical analysis, neither EBV latent or lytic cycle proteins nor C3d/EBV receptor were expressed by neoplastic cells. EBER positivity was significantly correlated with prominent lymphoid reaction (P = 0.0002) which was associated with numerous PS100-positive dendritic cells and with HLA-DR expression by tumour cells (P = 0.03). p53 immunoreactivity in more than 30% of tumour cells was detected in 25 out 49 EBV-negative cases and was absent in EBV-positive cases except in one case with focal EBER-positivity.
Focal staining for EBER is an unusual finding in the setting of gastric carcinoma and these results suggest that there might be two types of EBV-associated gastric carcinoma in which the viral infection will play a different role. The presence of a stromal lymphoid reaction which is strongly correlated with EBV positivity, is associated with antigen-presenting ability by HLA-DR-positive tumour cells or abundant dendritic cells. The function of p53 appears preserved in all EBV-associated carcinomas except in one case with focal EBER expression whereas the immunohistochemical pattern of p53 is suggestive of a mutational phenomenon in 51% of EBV-negative cases.
通过原位杂交检测EB病毒编码RNA（EBER），研究56例西方患者胃癌中EB病毒（EBV）的存在情况。比较EBV阳性和阴性胃癌的各种病理参数，包括p53过表达情况。
共7例检测到EBER转录本：52例传统型胃癌中有4例（7.7%），4例伴有淋巴间质的胃癌中有3例（75%）。5例传统型胃癌中EBER阳性呈弥漫性，2例传统型胃癌中局限于肿瘤局部区域。Southern印迹分析检测的病例显示为单克隆EBV基因组模式。免疫组化分析显示，肿瘤细胞既不表达EBV潜伏或裂解周期蛋白，也不表达C3d/EBV受体。EBER阳性与显著的淋巴反应显著相关（P = 0.0002），后者与大量PS100阳性树突状细胞以及肿瘤细胞HLA-DR表达相关（P = 0.03）。49例EBV阴性病例中有25例检测到超过30%的肿瘤细胞p53免疫反应性，EBV阳性病例中除1例局灶性EBER阳性外均未检测到。
在胃癌背景下，EBER局灶性染色是不常见的发现，这些结果提示可能存在两种类型的EBV相关胃癌，其中病毒感染发挥不同作用。与EBV阳性密切相关的间质淋巴反应的存在，与HLA-DR阳性肿瘤细胞或丰富树突状细胞的抗原呈递能力相关。除1例局灶性EBER表达的病例外，所有EBV相关癌中p53功能似乎均保留，而p53的免疫组化模式提示51%的EBV阴性病例存在突变现象。
