Jin A J, Nossal R
Laboratory of Physical Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Biophys J. 2000 Mar;78(3):1183-94. doi: 10.1016/S0006-3495(00)76676-8.
Statistical analysis is applied to a set of electron micrographic images (Kocsis, E., B. L. Trus, C. J. Steer, M. E. Bisher, and A. C. Steven. 1991. J. Struct. Biol. 107:6-14), from which quantitative measures are obtained to support the notion that the three arms of a triskelion have statistically identical properties and exhibit independent structural fluctuations. Additionally, a study of local contour fluctuations, which indicates that the elastic properties of a triskelion arm are approximately constant over the entire arm length, is used along with a small deformation statistical mechanics theory to derive an effective, average flexural rigidity for the arms. This result is used to estimate the bending energy necessary to deform a clathrin patch, and comparison is made with the deformation energy of an equivalent area of non-clathrin-coated membrane. We estimate that the rigidity of the clathrin lattice is at least comparable to that of a typical membrane. Hence, the natural curvature of a clathrin cage can stabilize, and perhaps propel, the formation of intracellular coated vesicles.
统计分析应用于一组电子显微图像（Kocsis, E., B. L. Trus, C. J. Steer, M. E. Bisher, and A. C. Steven. 1991. J. Struct. Biol. 107:6 - 14），从中获得定量测量结果，以支持三脚蛋白复合体的三条臂在统计上具有相同性质并表现出独立结构波动的观点。此外，对局部轮廓波动的研究表明，三脚蛋白复合体臂的弹性性质在整个臂长上大致恒定，该研究与小变形统计力学理论一起用于推导臂的有效平均弯曲刚度。该结果用于估计使网格蛋白斑块变形所需的弯曲能量，并与等效面积的非网格蛋白包被膜的变形能量进行比较。我们估计网格蛋白晶格的刚度至少与典型膜的刚度相当。因此，网格蛋白笼的自然曲率可以稳定并可能推动细胞内包被小泡的形成。
