修复错配DNA异源双链体中的修复片段。
Repair tracts in mismatched DNA heteroduplexes.
作者信息
Wagner R, Meselson M
出版信息
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4135-9. doi: 10.1073/pnas.73.11.4135.
Abstract
Heteroduplexes with mismatches at four sites were constructed from separated strands of lambda DNA and used to transfect Escherichia coli under recombinationless conditions. The output phages from 967 single cells in one experiment and 1016 in another were analyzed to determine the pattern of mismatch repair. A wide range of repair frequencies was found among the mismatches studied. Repair involving two or more close sites in the same heteroduplex occurs much more often on thesamestrandthanonopposite strands. Analysis of the pattern of repair suggeststhat repair tracts initiate at mismatches, propagate preferentially in the 5'leads to 3' direction, and extend an average distance of ca 3000 nucleotides.
摘要
利用λ噬菌体DNA分离出的链构建了在四个位点存在错配的异源双链体，并在无重组条件下用于转染大肠杆菌。对一个实验中967个单细胞以及另一个实验中1016个单细胞产生的子代噬菌体进行分析，以确定错配修复模式。在所研究的错配中发现了广泛的修复频率范围。同一异源双链体中两个或更多相邻位点的修复在同一条链上发生的频率远高于在相反链上。对修复模式的分析表明，修复片段起始于错配处，优先沿5'至3'方向延伸，平均延伸距离约为3000个核苷酸。
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