Wildenberg J, Meselson M
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2202-6. doi: 10.1073/pnas.72.6.2202.
DNA with base pair mismatches was prepared by annealing mixtures of genetically marked DNA from bacteriophage lambda. This heteroduplex DNA was used to transfect bacteria under conditions minimizing recombination. Genetic analysis of the progeny phages indicates that: (i) Mismatch repair occurs, usually giving rise to a DNA molecule with one chain with the genotype arising from repair and one parental chain. (ii) The frequency of repair of a given mismatch to wild type depends on the marker, ranging from 3 to 20%. (iii) Excision tracts may extend several hundred nucleotides but are usually shorter than about 2000 nucleotides. (iv) In Rec-mediated bacteriophage crosses, recombination of markers closer than about 10-3 nucleotide pairs frequently occurs by mismatch repair within heteroduplex DNA. (V) The average amount of heteroduplex DNA formed in a Rec-mediated recombination event is a few thousand nucleotide pairs.
通过对来自噬菌体λ的基因标记DNA混合物进行退火，制备出具有碱基对错配的DNA。在将重组减到最少的条件下，用这种异源双链DNA转染细菌。对后代噬菌体的遗传分析表明：（i）错配修复会发生，通常会产生一个DNA分子，其一条链具有修复产生的基因型，另一条链为亲本链。（ii）将给定错配修复为野生型的频率取决于标记，范围为3%至20%。（iii）切除片段可能延伸数百个核苷酸，但通常短于约2000个核苷酸。（iv）在Rec介导的噬菌体杂交中，距离小于约10⁻³核苷酸对的标记之间的重组经常通过异源双链DNA中的错配修复发生。（v）在Rec介导的重组事件中形成的异源双链DNA的平均量为几千个核苷酸对。
