Arnao M B, Cano A, Acosta M
Department of Plant Biology (Plant Physiology), University of Murcia, Spain.
Free Radic Res. 1999 Dec;31 Suppl:S89-96. doi: 10.1080/10715769900301371.
Methods to determine total antioxidant activity (TAA) are generally based on the inhibition of certain reactions by the presence of antioxidants. The most widely used methods are those that involve the generation of radical compounds, and it is the presence of antioxidants that determines the disappearance of these radicals. Strategies for measuring TAA are discussed, particularly the different methodological and instrumental approaches used. In addition, our own methods are presented in order to facilitate and speed up the manipulation of biological material. The values of TAA by different compounds are presented and compared.
测定总抗氧化活性(TAA)的方法通常基于抗氧化剂的存在对某些反应的抑制作用。最广泛使用的方法是那些涉及自由基化合物生成的方法,而抗氧化剂的存在决定了这些自由基的消失。本文讨论了测量TAA的策略,特别是所使用的不同方法学和仪器方法。此外,还介绍了我们自己的方法,以方便和加快生物材料的处理。给出并比较了不同化合物的TAA值。