López M, Seoane L, García M C, Lago F, Casanueva F F, Señarís R, Diéguez C
Department of Physiology, University of Santiago de Compostela, Santiago de Compostela, Spain.
Biochem Biophys Res Commun. 2000 Mar 5;269(1):41-5. doi: 10.1006/bbrc.2000.2245.
The aim of this study was to determine the effects of leptin treatment on prepro-orexin and orexin receptor expression in the rat hypothalamus. Adult male rats, food-deprived for 48 and 72 h, were treated one time with vehicle or leptin (10 microg, icv). Prepro-orexin mRNA content was measured by semiquantitative RT-PCR, Northern blot, and in situ hybridization; orexin receptor 1 and 2 mRNA content was quantified by Northern blot and/or semiquantitative RT-PCR. Our results indicate that leptin inhibits a fasting-induced increase in prepro-orexin mRNA and orexin receptor 1 mRNA levels in the rat hypothalamus, while orexin receptor 2 mRNA levels were unchanged in all situations evaluated. These data provide direct evidence for an additional mechanism of adaptation of the hypothalamus to food deprivation and for a new effect of leptin in the regulation of food intake.
本研究的目的是确定瘦素治疗对大鼠下丘脑前阿黑皮素原和阿黑皮素受体表达的影响。将成年雄性大鼠禁食48小时和72小时，然后一次性给予溶剂或瘦素（10微克，脑室内注射）。通过半定量逆转录聚合酶链反应（RT-PCR）、Northern印迹法和原位杂交法检测前阿黑皮素原mRNA含量；通过Northern印迹法和/或半定量RT-PCR法定量阿黑皮素受体1和2的mRNA含量。我们的结果表明，瘦素可抑制大鼠下丘脑前阿黑皮素原mRNA和阿黑皮素受体1 mRNA水平因禁食诱导的升高，而在所有评估情况下，阿黑皮素受体2 mRNA水平均未改变。这些数据为下丘脑适应食物剥夺的另一种机制以及瘦素在调节食物摄入方面的新作用提供了直接证据。
