Yamashita T, Hashimoto S, Kaneko S, Nagai S, Toyoda N, Suzuki T, Kobayashi K, Matsushima K
First Department of Internal Medicine & CREST, School of Medicine, University of Kanazawa, 13-1 Takara-Machi, Kanazawa, Ishikawa, 920-8641, Japan.
Biochem Biophys Res Commun. 2000 Mar 5;269(1):110-6. doi: 10.1006/bbrc.2000.2272.
To investigate the gene expression profile of a normal human liver, we performed serial analysis of gene expression (SAGE), which allows the quantitative and simultaneous analysis of thousands of genes expressed in tissue. Polyadenylated RNA was obtained from a bulk normal human liver sample and SAGE was performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) was also performed in each of 3 different normal liver samples to evaluate the validity of the profile in each individual. A total of 30,982 tags were sequenced, 8,596 of which were unique. The genes highly expressed in the normal liver were those encoding plasma proteins (>21.8% of total transcripts), cytoplasmic proteins (>8.6%), enzymes (>4.8%), protease inhibitors (>1.7%), complements (>1.1%), and coagulation factors (>0.75%). About 13.9% of all transcripts encoded genes not reported in GenBank thus far. This study identifies candidate genes to be examined in relation to various human liver diseases, including viral hepatitis, liver cirrhosis, and hepatocellular carcinoma.
为了研究正常人类肝脏的基因表达谱，我们进行了基因表达系列分析（SAGE），该方法能够对组织中数千个基因进行定量和同步分析。从一份正常人类肝脏样本中获取了聚腺苷酸化RNA，并进行了SAGE分析。还对3份不同的正常肝脏样本分别进行了逆转录聚合酶链反应（RT-PCR），以评估每个个体中基因表达谱的有效性。总共对30982个标签进行了测序，其中8596个是独特的。在正常肝脏中高表达的基因包括编码血浆蛋白的基因（占总转录本的21.8%以上）、细胞质蛋白的基因（占8.6%以上）、酶的基因（占4.8%以上）、蛋白酶抑制剂的基因（占1.7%以上）、补体的基因（占1.1%以上）和凝血因子的基因（占0.75%以上）。所有转录本中约13.9%的基因在GenBank中尚未报道。本研究确定了与各种人类肝脏疾病相关的待检测候选基因，包括病毒性肝炎、肝硬化和肝细胞癌。
