高效液相色谱法测定养心麝香丸中脂蟾毒配基和华蟾酥毒基的含量
Determination of resibufogenin and cinobufagin in heart-protecting musk pill by HPLC.
作者信息
Song H, Guo T, Bi K, Wang H, Zhang R
机构信息
Department of Pharmacy, The General Hospital of Shenyang Military Region, Shenyang 110015, People's Republic of China.
出版信息
Biomed Chromatogr. 2000 Apr;14(2):130-2. doi: 10.1002/(SICI)1099-0801(200004)14:2<130::AID-BMC929>3.0.CO;2-1.
A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of resibufogenin and cinobufagin in traditional Chinese medicine (heart-protecting musk pill, shexiang baoxin wan in Chinese) was developed. A reversed-phase system with a Hypersil (ODS2) C(18) column and tetrahydrofuran: methanol: water (8:31:61) mobile phase was employed for the separation of resibufogenin and cinobufagin. The detection was set at 299 nm and ethinyl estradiol was chosen as the internal standard. The limit of detection was 1.5 ng for resibufogenin and 2.0 ng for cinobufagin at a signal-to-noise ratio of 4:1. It is a rapid, simple and accurate method for quantitative analysis of resibufogenin and cinobufagin in heart-protecting musk pill.
建立了一种高效液相色谱（HPLC）法，用于同时测定中成药（护心麝香丸，中文名为麝香保心丸）中脂蟾毒配基和华蟾酥毒基的含量。采用Hypersil（ODS2）C18柱和四氢呋喃：甲醇：水（8:31:61）流动相的反相系统分离脂蟾毒配基和华蟾酥毒基。检测波长设定为299nm，选择炔雌醇作为内标。在信噪比为4:1时，脂蟾毒配基的检测限为1.5ng，华蟾酥毒基的检测限为2.0ng。该方法是一种快速、简便、准确的定量分析护心麝香丸中脂蟾毒配基和华蟾酥毒基的方法。
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