[高效液相色谱法测定蟾酥中脂蟾毒配基和华蟾酥毒基的含量]
[Determination of resibufogenin and cinobufagin in venenum Bufonis by HPLC].
作者信息
Wang J, Jin W, Jin H, Zhou F
机构信息
Beijing Municipal Institute for Drug Control, Beijing 100035.
出版信息
Zhongguo Zhong Yao Za Zhi. 1998 Nov;23(11):651-3, 702.
OBJECTIVE
To establish a HPLC method to determine the contents of active constituents in Venenum Bufonis.
METHOD
In determining the contents of resibufogenin and cinobufagin in the traditional Chinese medicine Venenum Bufonis, the mobile phase was acetonitrile-0.5% KH2PO4 solution(50:50)(pH adjusted with value phosphoric acid to 3.25 +/- 0.02).
RESULT
The constituents thus determined have good linearity and separation. The average recovery of resibufogenin was 100.35%, RSD 1.86%; the average recovery of cinobufagin was 100.38%, RSD 2.09%.
CONCLUSION
The method was convenient, rapid, accurate and practicable.
目的
建立一种高效液相色谱法测定蟾酥中活性成分的含量。
方法
在测定中药蟾酥中脂蟾毒配基和华蟾酥毒基的含量时，流动相为乙腈-0.5%磷酸二氢钾溶液（50:50）（用磷酸调pH值至3.25±0.02）。
结果
所测成分具有良好的线性关系和分离度。脂蟾毒配基的平均回收率为100.35%，相对标准偏差为1.86%；华蟾酥毒基的平均回收率为100.38%，相对标准偏差为2.09%。
结论
该方法简便、快速、准确、可行。
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