Weichselbaum R R, Epstein J, Little J B, Kornblith P
AJR Am J Roentgenol. 1976 Dec;127(6):1027-32. doi: 10.2214/ajr.127.6.1027.
It is well known that radiation therapy can be successfully used to cure or control some types of human tumors, while consistently failing in others. This has been ascribed to several factors including differences in the intrinsic sensitivity of the tumor cells and in their ability to recover from radiation damage. In this study, human tumor cells from an osteogenic sarcoma, a glioblastoma, and two medulloblastomas, as well as cells from human skin, were established in tissue culture, and the in vitrox x-ray survival and DNA repair parameters determined. No significant differences in either clonogenic survival or DNA strand rejoining ability could be detected among these human tumors or skin cells, despite the wide variability in their radiocurability in vivo. In addition, skin cell strains derived from patients exhibiting markedly sensitive or resistant skin reactions during fractionated radiotherapy showed no differences in survival characteristics from normal controls. It is therefore suggested that the wide range of radiocurabilities seen among various human tumors cannot be explained on the basis of inherent cellular factors responsible for the survival of tumor cells after x-irradiation.
众所周知，放射治疗能够成功用于治愈或控制某些类型的人类肿瘤，但在其他一些肿瘤上却始终失败。这归因于多种因素，包括肿瘤细胞内在敏感性及其从辐射损伤中恢复能力的差异。在本研究中，从骨肉瘤、胶质母细胞瘤和两个髓母细胞瘤中获取的人类肿瘤细胞以及人类皮肤细胞被建立于组织培养中，并测定了体外X射线存活和DNA修复参数。尽管这些人类肿瘤或皮肤细胞在体内的放射可治愈性差异很大，但在克隆形成存活或DNA链重连能力方面均未检测到显著差异。此外，在分次放射治疗期间表现出明显敏感或抗性皮肤反应的患者所衍生的皮肤细胞系，其存活特征与正常对照无差异。因此，有人提出，各种人类肿瘤之间所观察到的广泛放射可治愈性差异，无法基于负责X射线照射后肿瘤细胞存活的内在细胞因素来解释。
