Barouch R, Appel E, Kazimirsky G, Braun A, Renz H, Brodie C
Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
J Neuroimmunol. 2000 Mar 1;103(2):112-21. doi: 10.1016/s0165-5728(99)00233-7.
In this study, we examined the expression of neurotrophins in mouse lymphocytes and the regulation of their expression by mitogens and neurotransmitters. We found that mixed splenocytes as well as T and B lymphocytes expressed mRNA for all the neurotrophins examined. Differential regulation of the neurotrophins was obtained upon stimulation of the cells. Thus, LPS increased the expression of NGF, BDNF and NT-3 in splenocytes and B cells, whereas Con-A increased the mRNA of NT-3 and NT-4 in T cells and NGF expression in splenocytes. The neurotransmitter substance P and the beta-adrenergic agonist, isoproterenol induced an increase in the expression of NGF. Our results suggest an important role for the different neurotrophins in the function of the immune system and point to a bi-directional interaction between neurotrophins and neurotransmitters in this system.
在本研究中，我们检测了神经营养因子在小鼠淋巴细胞中的表达以及丝裂原和神经递质对其表达的调控。我们发现混合脾细胞以及T淋巴细胞和B淋巴细胞都表达所检测的所有神经营养因子的mRNA。细胞受到刺激后，神经营养因子呈现出差异调控。因此，脂多糖（LPS）增加了脾细胞和B细胞中神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养因子-3（NT-3）的表达，而刀豆蛋白A（Con-A）增加了T细胞中NT-3和NT-4的mRNA以及脾细胞中NGF的表达。神经递质P物质和β-肾上腺素能激动剂异丙肾上腺素可诱导NGF表达增加。我们的结果表明不同的神经营养因子在免疫系统功能中发挥重要作用，并指出该系统中神经营养因子与神经递质之间存在双向相互作用。
