Vilanova M, Tavares D, Ferreira P, Oliveira L, Nóbrega A, Appelberg R, Arala-Chaves M
Department of Immunology, Institute for Biomedical Sciences Abel Salazar, Porto, Portugal.
Scand J Immunol. 1996 Feb;43(2):155-63. doi: 10.1046/j.1365-3083.1996.d01-25.x.
CD69 is an early marker of lymphoid cell activation. The authors report on an up-regulation of CD69 in splenic B and T cells of C57Bl/6 mice after administration of lipopolysaccharide (LPS) or microbial immunosuppressive/mitogenic (ISM) proteins produced by C. albicans (p43) and African Swine Fever Virus (p36). This up-regulation of CD69 was observed 6 and 24 h after mitogenic treatments. The same pattern of increased CD69 expression was observed in the lymph nodes of mice treated with p43 or LPS, whereas p36 treatment failed to induce increased CD69 expression in this organ. Intracellular calcium mobilization was induced in splenic B and T lymphocytes after incubation of total spleen cells with LPS, p43 or p36. This increase was higher in B than in T cells. Increased calcium mobilization was also seen in lymph node B cells after incubation with p43 or p36 and in lymph node T cells after p43 stimulation. Up-regulation of CD69 expression on B and T cells was also observed after in vitro stimulation of spleen cells with the three mitogens used. Similar results were obtained with culture supernatants of macrophage/monocyte (M phi) cells activated with LPS (LPS/M phi CS). Stimulation of M phi cells with LPS or with the ISM proteins is demonstrated by the increased production of nitrites by these cells. The increased in vitro expression of CD69 was, however, not abolished by monoclonal antibodies to M phi cytokines such as IL-6, IL-10 or TNF alpha. No increased expression of CD69 was found in vitro on purified B or T cells, even when mixed upon stimulation with p43, p36, LPS or with LPS/M phi CS. However, an increase in the expression of CD69 was observed on B cells co-cultured with M phi cells after treatment with LPS or p36. All three mitogens failed to induce increased CD69 expression on cultured T cells mixed with M phi cells.
CD69是淋巴细胞活化的早期标志物。作者报告称，在给C57Bl/6小鼠注射脂多糖（LPS）或白色念珠菌（p43）和非洲猪瘟病毒（p36）产生的微生物免疫抑制/促有丝分裂（ISM）蛋白后，其脾脏B细胞和T细胞中CD69上调。在促有丝分裂处理后6小时和24小时观察到这种CD69上调。在用p43或LPS处理的小鼠淋巴结中观察到相同的CD69表达增加模式，而p36处理未能诱导该器官中CD69表达增加。在用LPS、p43或p36孵育全脾细胞后，脾脏B淋巴细胞和T淋巴细胞中诱导了细胞内钙动员。这种增加在B细胞中比在T细胞中更高。在用p43或p36孵育后，淋巴结B细胞中也观察到钙动员增加，在p43刺激后，淋巴结T细胞中也观察到钙动员增加。在用三种促有丝分裂原体外刺激脾细胞后，也观察到B细胞和T细胞上CD69表达上调。用LPS激活的巨噬细胞/单核细胞（M phi）细胞的培养上清液（LPS/M phi CS）也获得了类似结果。用LPS或ISM蛋白刺激M phi细胞可通过这些细胞中亚硝酸盐产量增加来证明。然而，针对M phi细胞因子如IL-6、IL-10或TNFα的单克隆抗体并未消除体外CD69表达的增加。在纯化的B细胞或T细胞上，即使在用p43、p36、LPS或LPS/M phi CS刺激时混合，也未发现体外CD69表达增加。然而，在用LPS或p36处理后，与M phi细胞共培养的B细胞上观察到CD69表达增加。所有三种促有丝分裂原均未能在与M phi细胞混合培养的T细胞上诱导CD69表达增加。
