Coletta R D, Almeida O P, Reynolds M A, Sauk J J
Department of Oral Pathology, Dental School, University of Campinas Piracicaba, São Paulo, Brazil.
J Periodontal Res. 1999 Nov;34(8):457-63. doi: 10.1111/j.1600-0765.1999.tb02281.x.
Hereditary gingival fibromatosis (HGF) is characterized by an excess accumulation of extracellular matrix (ECM) resulting in a generalized and fibrotic enlargement of the gingiva. To investigate some of the regulatory features of this condition, gingival fibroblasts from normal gingiva (NG) and HGF were examined for the expression and production of matrix metalloproteinases (MMPs) and their inhibitors, tissue matrix metalloproteinases inhibitor (TIMPs). Our results, obtained from 2 different assays, semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography, clearly demonstrated that the expression and production of MMP-1 and MMP-2 was significantly lower in fibroblasts from HGF than from NG. Interestingly, TIMP-1 and TIMP-2 expression from NG cells was shown to be slightly higher to those from HGF. Addition of antibodies against transforming growth factor-beta 1 (TGF-beta 1), which is produced in greater amounts by HGF fibroblasts, resulted in a slight increase in MMP-1 and a decrease in MMP-2 expression, whereas TIMP-1 and TIMP-2 expressions were unaffected. These patterns of expression and production suggest that enhanced TGF-beta 1 production reduce the proteolytic activities of HGF fibroblasts, which favor the accumulation of ECM.
遗传性牙龈纤维瘤病（HGF）的特征是细胞外基质（ECM）过度积聚，导致牙龈普遍纤维化肿大。为了研究这种病症的一些调节特征，对来自正常牙龈（NG）和HGF的牙龈成纤维细胞进行了基质金属蛋白酶（MMPs）及其抑制剂组织基质金属蛋白酶抑制剂（TIMPs）的表达和产生情况的检测。我们通过两种不同的检测方法，即半定量逆转录聚合酶链反应（RT-PCR）和酶谱分析获得的结果清楚地表明，HGF成纤维细胞中MMP-1和MMP-2的表达和产生明显低于NG成纤维细胞。有趣的是，NG细胞中TIMP-1和TIMP-2的表达略高于HGF细胞。添加针对转化生长因子-β1（TGF-β1）的抗体（HGF成纤维细胞产生的TGF-β1量更多），导致MMP-1略有增加，MMP-2表达减少，而TIMP-1和TIMP-2的表达未受影响。这些表达和产生模式表明，TGF-β1产生的增加降低了HGF成纤维细胞的蛋白水解活性，这有利于ECM的积累。
