Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Parasitol Res. 2010 Aug;107(3):509-16. doi: 10.1007/s00436-010-1883-1. Epub 2010 Apr 29.
Electrophoretic protein profiles of female salivary glands of five sibling species within the Anopheles barbirostris complex, namely A. barbirostris species A1 (Forms A, B, and D), A2, A3, and A4 and Anopheles campestris-like (Forms B and E), were analyzed. At least eight major and several minor protein bands were detected in the glands of each species, of which each morphological region contained different major proteins. The protein profiles distinguished the five sibling species. The variability in major proteins among species was observed in the 40-48, 32-37, and 10-18 kDa ranges. No difference in protein profiles was found in different cytogenetic forms. Polymorphism of the protein profiles within species was only noted in species A4. The lowest major protein (marker) band of each species showed remarkably different relative mobility on SDS-polyacrylamide gels. NanoLC-MS analysis revealed that the marker protein of some species matched with a protein involving in blood feeding, gSG6, of Anopheles gambiae and Anopheles freeborni. These results might be useful for construction of an additional tool to distinguish the five sibling species and lead to further study on the evolution of blood feeding and pathogen transmission.
对按蚊复组中 5 个姐妹种的雌性唾液腺的电泳蛋白图谱进行了分析，即按蚊巴尔比罗斯特斯种 A1（A、B 和 D 型）、A2、A3、A4 和类坎氏按蚊（B 和 E 型）。在每个种的腺体中至少检测到 8 条主要蛋白带和几条次要蛋白带，其中每个形态区域都含有不同的主要蛋白。蛋白图谱可区分 5 个姐妹种。在 40-48、32-37 和 10-18 kDa 范围内观察到种间主要蛋白的变异性。不同细胞遗传学形式的蛋白图谱没有差异。仅在 A4 种中观察到种内蛋白图谱的多态性。每个种的最低主要蛋白（标记）带在 SDS-聚丙烯酰胺凝胶上的相对迁移率明显不同。NanoLC-MS 分析表明，一些种的标记蛋白与涉及吸血的 gSG6 蛋白，即冈比亚按蚊和自由伯氏按蚊的蛋白相匹配。这些结果可能有助于构建一种额外的工具来区分这 5 个姐妹种，并进一步研究吸血和病原体传播的进化。
