Host-Parasite Interaction Biology Group, National Institute of Malaria Research, Sector-8, Dwarka, Delhi, India.
J Insect Physiol. 2011 Oct;57(10):1399-406. doi: 10.1016/j.jinsphys.2011.07.007. Epub 2011 Jul 20.
In this study, we analyzed a small scale transcriptome of salivary glands in sugar fed female mosquitoes. Thirty five percent of the transcripts could not be assigned a function. Some of them may code for salivary gland specific products involved in sugar feeding. We identified and characterized two new putative cDNAs encoding a sugar transporter and a cAMP generating DAPIT (Diabetes-Associated proteins in insulin sensitive tissues). Down regulation of these two cDNAs in response to blood feeding suggest that both AsST and AsDAPIT salivary genes may specifically be involved in the facilitation of sugar metabolism and energy production. The inability to absorb or digest sugar may cause organ failure, improper functioning of nervous system, behavioral disorder and death. Further functional characterization of theses putative transcripts is under investigation to examine their role in the mosquito salivary glands.
在这项研究中，我们分析了喂食糖的雌性蚊子的小范围唾液腺转录组。其中 35%的转录本无法被赋予功能。它们中的一些可能编码与糖摄入有关的唾液腺特异性产物。我们鉴定并表征了两个新的假定 cDNA，它们编码一个糖转运体和一个 cAMP 生成的 DAPIT（胰岛素敏感组织中的糖尿病相关蛋白）。这两个 cDNA 在吸血后的下调表明，AsST 和 AsDAPIT 唾液基因可能专门参与促进糖代谢和能量产生。不能吸收或消化糖可能导致器官衰竭、神经系统功能异常、行为障碍和死亡。为了研究它们在蚊子唾液腺中的作用，我们正在进一步对这些假定的转录本进行功能表征。
