Avunduk A M, Yardimci S, Avunduk M C, Kurnaz L, Cengiz M
Department of Ophthalmology, Karadeniz Technical University, School of Medicine, Trabzon, Turkey.
Jpn J Ophthalmol. 2000 Jan-Feb;44(1):88-91. doi: 10.1016/s0021-5155(99)00169-0.
X-ray and other radiation can cause cataract, but the pathogenic mechanism is largely unknown. The aim of this study was to investigate the accumulation of iron in the x-ray-exposed rat lens and its relationship to lens injury.
Fifty male Wistar rats were divided randomly into five groups of 10. Groups 2 and 4 rats were sham-exposed, groups 3 and 5 were x-ray-treated, and group 1 served as control. X-ray radiation and sham exposure were performed in a similar manner. After 10 and 30 days of exposure, a lens from each rat in groups 2 and 3, and 3 and 5, respectively, were analyzed by flame atomic absorption technique for the assessment of metal content.
Significantly decreased zinc and increased iron and calcium concentrations were detected in the lens samples of groups 3 and 5 compared with groups 2 and 4 and controls. Similar results were obtained comparing groups 5 and 3.
We propose that x-ray exposure may cause toxic cell injury of the rat lens via Fenton metals catalyzed damage. Initial lens membrane damage in the radiolytic phase may permit the access of iron resulting in lens damage.
X射线及其他辐射可导致白内障，但其致病机制 largely unknown。本研究旨在探究X射线照射大鼠晶状体中铁的蓄积情况及其与晶状体损伤的关系。
50只雄性Wistar大鼠随机分为5组，每组10只。第2组和第4组大鼠进行假照射，第3组和第5组进行X射线处理，第1组作为对照。X射线照射和假照射以相似方式进行。照射10天和30天后，分别对第2组和第3组、第3组和第5组的每只大鼠的晶状体采用火焰原子吸收技术进行分析，以评估金属含量。
与第2组、第4组及对照组相比，第3组和第5组的晶状体样本中锌含量显著降低，铁和钙浓度升高。比较第5组和第3组也获得了相似结果。
我们认为，X射线照射可能通过芬顿金属催化损伤导致大鼠晶状体毒性细胞损伤。辐射分解阶段晶状体膜的初始损伤可能使铁进入，从而导致晶状体损伤。
