Department of Animal Science, Faculty of Ahar Agriculture & Natural Resources, University of Tabriz, Tabriz, Iran.
Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Arch Razi Inst. 2021 Dec 30;76(6):1787-1795. doi: 10.22092/ari.2021.355960.1765. eCollection 2021 Dec.
Current experiment was carried out in factorial 2×2 arrangement to study the effects of stress (with or without dexamethasone administration) and addition of dietary selenium (with or without selenium supplementation in the diet) in male broiler breeder on the quality of frozen-thawed sperm under oxidative stress induced by dexamethasone. A total of 24 broiler breeder roosters with the age of 28 weeks were used based on a completely randomized design with four therapeutic approaches (factorial 2×2) and six birds in each approach. The experimental treatments were: 1) basal diet without selenium supplementation and injection of saline (CON), 2) basal diet with dexamethasone injection (4 mg/kg BW, three times every other day for one week), (DEX), 3) without dexamethasone injection and supplementation with 0.3 mg/kg selenium (Sel-Plex), and 4) dexamethasone injection and basal diet supplemented with 0.3 mg/kg of diet selenium (Sel-Plex+Dex). Sperm samples were collected from roosters. Motility, progressive motility, plasma membrane integrity, viability, malondialdehyde concentration and antioxidant parameters were evaluated in fresh and frozen-thawed semen. In spite of non-significant interaction effects, factorial analysis indicated the significant effect of every factor on different experimental parameters in fresh and frozen-thawed semen (<0.05); The results revealed that total and progressive motility, plasma membrane integrity and viability were lower in DEX group when compared with other treatments (<0.05). On the other hand, malondialdehyde concentration was higher in DEX group in comparison with Con, Sel-Plex and Sel-Plex+DEX groups (<0.05). Moreover, total antioxidant capacity, level of glutathione peroxidase and superoxide dismutase were lower in DEX group as compared with other treatments (<0.05). Our findings indicated that administration of selenium in dexamethasone-receiving roosters (Sel-Plex+DEX) improved the parameters of fresh and frozen-thawed sperm; but the best results were observed in Sel-Plex treatment. Therefore, selenium supplementation in the diet of roosters without dexamethasone injection improved total motility, progressive motility, membrane integrity, viability, malondialdehyde, total antioxidant capacity, glutathione peroxidase and superoxide dismutase pre- and post-freezing. It can be concluded, selenium in organic forms in stressed and non-stressed rooster's diet might improve all motility and antioxidant parameters in fresh and frozen-thawed sperm.
本实验采用 2×2 析因设计，研究应激（给予或不给予地塞米松）和膳食硒添加（给予或不给予饮食硒补充）对 28 周龄雄性种公鸡冷冻-解冻精子质量的影响，在应激诱导的地塞米松下。共使用 24 只雄性种公鸡，采用完全随机设计，有 4 种治疗方法（析因 2×2），每种方法 6 只。实验处理为：1）基础日粮，不添加硒，注射生理盐水（CON），2）基础日粮，地塞米松注射（4mg/kgBW，每两天一次，连续一周），（DEX），3）不注射地塞米松，补充 0.3mg/kg 硒（Sel-Plex），4）地塞米松注射，基础日粮添加 0.3mg/kg 饮食硒（Sel-Plex+Dex）。从公鸡中采集精子样本。评价新鲜和冷冻-解冻精液的运动性、渐进性运动、质膜完整性、活力、丙二醛浓度和抗氧化参数。尽管没有显著的互作效应，但析因分析表明，每个因素对新鲜和冷冻-解冻精液中不同实验参数的影响均有显著差异（<0.05）；结果表明，DEX 组的总运动率、渐进性运动率、质膜完整性和活力均低于其他处理组（<0.05）。另一方面，DEX 组的丙二醛浓度高于 Con、Sel-Plex 和 Sel-Plex+DEX 组（<0.05）。此外，DEX 组的总抗氧化能力、谷胱甘肽过氧化物酶和超氧化物歧化酶水平均低于其他处理组（<0.05）。我们的研究结果表明，在接受地塞米松的公鸡中添加硒（Sel-Plex+DEX）可改善新鲜和冷冻-解冻精子的参数；但在 Sel-Plex 处理中观察到的效果最好。因此，在不注射地塞米松的公鸡日粮中添加硒可提高冷冻前和冷冻后的总运动率、渐进性运动率、膜完整性、活力、丙二醛、总抗氧化能力、谷胱甘肽过氧化物酶和超氧化物歧化酶。可以得出结论，在应激和非应激公鸡的饮食中以有机形式添加硒可能会提高新鲜和冷冻-解冻精子的所有运动和抗氧化参数。
