Chan E Y, Mok T M, Lawton J W, Ko O K, Ho L, Lau C S
Department of Pathology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
Asian Pac J Allergy Immunol. 1999 Dec;17(4):275-9.
Anti-extractable nuclear antigen (ENA) antibodies were assayed by counter immunoelectrophoresis (CIE) and immunoblotting in patients with systemic lupus erythematosus (SLE). We found the two methods showed good concordance rates, the lowest being 67% for anti-SS-A. Immunoblotting was more sensitive in detecting anti-Sm, anti-SS-B and anti-PCNA (proliferating cell nuclear antigen); CIE was more sensitive for anti-nRNP and anti-SS-A. Overall, the prevalence of these anti-ENA antibodies in SLE was increased by 9-20% if immunoblotting was used in addition to CIE. Sera specific for the 52 kDa peptide of the SS-A antigen (anti-52kDa SS-A) were better detected by immunoblotting. Anti-PCNA antibody was found in 6.3% of SLE patients and was associated with active disease and hemolytic anemia. The positive rate of anti-Sm was 9% by CIE and 23.7% by immunoblotting and this antibody was a specific marker for SLE using either method. It was concluded that using immunoblotting in addition to CIE, the overall sensitivity of detection of anti-ENA antibodies in SLE was increased and clinically useful antibodies such as anti-52kDa SS-A and anti-PCNA could be detected.
采用对流免疫电泳（CIE）和免疫印迹法检测系统性红斑狼疮（SLE）患者的抗可提取核抗原（ENA）抗体。我们发现这两种方法的符合率良好，抗SS - A的最低符合率为67%。免疫印迹法在检测抗Sm、抗SS - B和抗增殖细胞核抗原（PCNA）方面更敏感；CIE对抗nRNP和抗SS - A更敏感。总体而言，如果除CIE外还使用免疫印迹法，SLE患者中这些抗ENA抗体的患病率会增加9% - 20%。免疫印迹法能更好地检测出针对SS - A抗原52 kDa肽段的血清（抗52kDa SS - A）。6.3%的SLE患者中发现了抗PCNA抗体，其与疾病活动和溶血性贫血相关。CIE检测抗Sm的阳性率为9%，免疫印迹法为23.7%，无论使用哪种方法，该抗体都是SLE的特异性标志物。得出的结论是，除CIE外使用免疫印迹法可提高SLE患者抗ENA抗体检测的总体敏感性，并能检测出如抗52kDa SS - A和抗PCNA等临床上有用的抗体。
