Williams D G, Charles P J, Maini R N
Division of Clinical Immunology, Mathilda and Terence Kennedy Institute of Rheumatology, London, U.K.
J Immunol Methods. 1988 Oct 4;113(1):25-35. doi: 10.1016/0022-1759(88)90378-x.
The p67 (67 kDa) and A (33 kDa) polypeptides of nRNP/Sm antigen and the B, B' (28 and 29 kda) and D (16 kDa) polypeptides of 'free' Sm antigen were isolated and used in enzyme-linked immunoadsorbent assays (ELISA) for human autoantibodies. ELISA specificity was demonstrated using monoclonal antibodies. The ELISA using HPLC-purified polypeptides was found to be more sensitive than immunoblotting for detecting antibody. 86% of sera with precipitating anti-nRNP antibodies were positive in the ELISA, as were all sera with precipitating anti-Sm antibodies. Patients with rheumatoid arthritis (RA), Sjögrens syndrome (SS) and undifferentiated connective tissue disease (UCTD) had low levels of anti-p67 with a prevalence 11.6% and 18%, respectively, whilst patients with systemic lupus erythematosus (SLE) and mixed connective tissue disease (MCTD) had high levels and prevalence rates of 55.2% and 80%, respectively. Anti-B or anti-D antibodies were detected at high levels in SLE (prevalence 30%) but were found rarely in UCTD and MCTD (prevalence 7% and 10%) and not at all in RA or SS sera.
分离出核糖核蛋白/史密斯（nRNP/Sm）抗原的p67（67 kDa）和A（33 kDa）多肽以及“游离”Sm抗原的B、B'（28和29 kDa）和D（16 kDa）多肽，并将其用于检测人自身抗体的酶联免疫吸附测定（ELISA）。使用单克隆抗体证明了ELISA的特异性。发现使用高效液相色谱（HPLC）纯化多肽的ELISA在检测抗体方面比免疫印迹更敏感。86%有沉淀性抗nRNP抗体的血清在ELISA中呈阳性，所有有沉淀性抗Sm抗体的血清也呈阳性。类风湿关节炎（RA）、干燥综合征（SS）和未分化结缔组织病（UCTD）患者的抗p67水平较低，患病率分别为11.6%和18%，而系统性红斑狼疮（SLE）和混合性结缔组织病（MCTD）患者的抗p67水平较高，患病率分别为55.2%和80%。在SLE中高水平检测到抗B或抗D抗体（患病率30%），但在UCTD和MCTD中很少发现（患病率7%和10%），在RA或SS血清中则完全未检测到。
