Lei H, Peeling J
Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, R3E 0W3, Canada.
J Magn Reson. 2000 Mar;143(1):95-100. doi: 10.1006/jmre.1999.1958.
Conventional double quantum (DQ) editing techniques recover resonances of one metabolite at a time and are thus inefficient for monitoring metabolic changes involving several metabolites. A DQ coherence transfer double editing sequence using a dual-band DQ coherence read pulse is described here. The sequence permits simultaneous spectral editing for two metabolites with similar J coupling constants in a single scan. Simultaneous editing for taurine and gamma-aminobutyric acid (GABA) is demonstrated using solution phantoms and rat brain tissue. Selectivity of the double editing sequence for the target metabolites is as good as that achieved using conventional DQ editing which selects each metabolite individually. With experimental parameters of the double editing sequence chosen to optimize GABA editing, the sensitivity for GABA detection is the same as that with GABA editing only, while the sensitivity for taurine detection is decreased slightly compared to that with taurine editing only.
传统的双量子（DQ）编辑技术每次只能恢复一种代谢物的共振信号，因此在监测涉及多种代谢物的代谢变化时效率较低。本文描述了一种使用双频DQ相干读取脉冲的DQ相干转移双编辑序列。该序列允许在单次扫描中对具有相似J耦合常数的两种代谢物进行同时光谱编辑。使用溶液模型和大鼠脑组织证明了对牛磺酸和γ-氨基丁酸（GABA）的同时编辑。双编辑序列对目标代谢物的选择性与传统DQ编辑（单独选择每种代谢物）所达到的选择性一样好。选择双编辑序列的实验参数以优化GABA编辑时，GABA检测的灵敏度与仅进行GABA编辑时相同，而牛磺酸检测的灵敏度与仅进行牛磺酸编辑时相比略有下降。
