Kunene N F, Geornaras I, von Holy A, Hastings J W
School of Molecular and Cellular Biosciences, University of Natal, Scottsville, 3209, South Africa.
Appl Environ Microbiol. 2000 Mar;66(3):1084-92. doi: 10.1128/AEM.66.3.1084-1092.2000.
The group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known, and these organisms have been characterized extensively by using different techniques. In this study, 180 lactic acid bacterial strains isolated from sorghum powder (44 strains) and from corresponding fermented (93 strains) and cooked fermented (43 strains) porridge samples that were prepared in 15 households were characterized by using biochemical and physiological methods, as well as by analyzing the electrophoretic profiles of total soluble proteins. A total of 58 of the 180 strains were Lactobacillus plantarum strains, 47 were Leuconostoc mesenteroides strains, 25 were Lactobacillus sake-Lactobacillus curvatus strains, 17 were Pediococcus pentosaceus strains, 13 were Pediococcus acidilactici strains, and 7 were Lactococcus lactis strains. L. plantarum and L. mesenteroides strains were the dominant strains during the fermentation process and were recovered from 87 and 73% of the households, respectively. The potential origins of these groups of lactic acid bacteria were assessed by amplified fragment length polymorphism fingerprint analysis.
包括乳酸菌在内的这组细菌是已知的最多样化的细菌群体之一，并且已经通过使用不同技术对这些微生物进行了广泛的表征。在本研究中，采用生化和生理学方法以及分析总可溶性蛋白的电泳图谱，对从15个家庭制备的高粱粉（44株）、相应的发酵粥（93株）和煮熟发酵粥（43株）样品中分离出的180株乳酸菌菌株进行了表征。180株菌株中，共有58株为植物乳杆菌菌株，47株为肠膜明串珠菌菌株，25株为清酒乳杆菌-弯曲乳杆菌菌株，17株为戊糖片球菌菌株，13株为嗜酸片球菌菌株，7株为乳酸乳球菌菌株。植物乳杆菌和肠膜明串珠菌菌株是发酵过程中的优势菌株，分别从87%和73%的家庭中分离得到。通过扩增片段长度多态性指纹分析评估了这些乳酸菌群体的潜在来源。
