Kondo H, Kazuta Y, Saito A, Fuji K
Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Japan.
Gene. 1997 May 20;191(1):47-50. doi: 10.1016/s0378-1119(97)00027-9.
A gene for prokaryotic pyruvate carboxylase (PC) was cloned from Bacillus stearothermophilus. It has an open reading frame of 3441 base pairs which can code for a protein of 128,353 Da. Not only the molecular size and domain organization but also the deduced amino acid sequence of B. stearothermophilus PC are similar to those of eukaryotic PCs.