Ziegler H, Muranyi W, Burgert H G, Kremmer E, Koszinowski U H
Max-von-Pettenkofer Institut, Genzentrum, Ludwig-Maximilians-Universität München, Germany.
EMBO J. 2000 Mar 1;19(5):870-81. doi: 10.1093/emboj/19.5.870.
Murine cytomegalovirus (MCMV) interferes with the MHC class I pathway of antigen presentation. The type I transmembrane glycoprotein gp40, encoded by the gene m152, retains major histocompatibility complex (MHC) class I complexes in the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC)/cis-Golgi. These MHC class I complexes are stable, show an extended half-life and do not exchange beta(2)-microglobulin, whereas gp40 reaches an endosomal/lysosomal compartment where it subsequently is degraded. The analysis of regions within the viral protein that are essential for MHC class I retention revealed that a secreted form of gp40, lacking the cytoplasmic tail and the transmembrane region, still has the capacity to retain MHC class I complexes. Continuous expression of gp40 is not required for MHC class I retention. Our data indicate that the retention of MHC class I complexes in the ERGIC/cis-Golgi is triggered by gp40 and does not require the further presence of the viral protein.
小鼠巨细胞病毒（MCMV）干扰抗原呈递的MHC I类途径。由基因m152编码的I型跨膜糖蛋白gp40将主要组织相容性复合体（MHC）I类复合体保留在内质网（ER）-高尔基体中间区室（ERGIC）/顺式高尔基体中。这些MHC I类复合体是稳定的，具有延长的半衰期，并且不交换β2-微球蛋白，而gp40到达内体/溶酶体区室，随后在那里被降解。对病毒蛋白中对于MHC I类保留至关重要的区域的分析表明，缺乏细胞质尾巴和跨膜区域的分泌形式的gp40仍然具有保留MHC I类复合体的能力。MHC I类保留不需要gp40的持续表达。我们的数据表明，gp40触发了MHC I类复合体在ERGIC/顺式高尔基体中的保留，并且不需要病毒蛋白的进一步存在。
