Sarthy P V, Meselson M
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4613-7. doi: 10.1073/pnas.73.12.4613.
Single bursts from three-point crosses of bacteriophage lambda were analyzed for recombination of markers several thousand nucleotide pairs apart. Single recombination, mediated by the rec system of Escherichia coli, is usually reciprocal. Double recombinants are also significantly correlated in single bursts, although the correlation is weaker than for reciprocal singles. Reciprocity is not found in crosses mediated by the lambda red system. With respect to certain other paraments of recombination, the two systems appear to be alike. Double recombinants usually result from one event, not from two independent single recombinations, and their average clone size is about half that of single recombinants. The results are discussed in terms of current molecular models of recombination.
对噬菌体λ三点杂交的单脉冲进行分析，以研究相隔数千个核苷酸对的标记的重组情况。由大肠杆菌rec系统介导的单重组通常是相互的。在单脉冲中，双重组体也显著相关，尽管这种相关性比相互单重组体的弱。在由λred系统介导的杂交中未发现相互性。就重组的某些其他参数而言，这两个系统似乎相似。双重组体通常由一个事件产生，而非两个独立的单重组产生，并且它们的平均克隆大小约为单重组体的一半。根据当前的重组分子模型对结果进行了讨论。
