一种用于辅助辣椒中番茄斑萎病毒（TSWV）抗性选择的CAPS标记。
A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper.
作者信息
Moury B, Pflieger S, Blattes A, Lefebvre V, Palloix A
机构信息
Institut National de la Recherche Agronomique (INRA), Unité de génétique et amélioration des fruits et légumes, Montfavet, France.
出版信息
Genome. 2000 Feb;43(1):137-42.
The hypersensitive resistance to tomato spotted wilt virus (TSWV) in pepper is determined by a single dominant gene (resistant allele: Tsw) in several Capsicum chinense genotypes. In order to facilitate the selection for this resistance, four RAPD (among 250 10-mer primers tested) were found linked to the Tsw locus using the bulked segregant analysis and 153 F2 individuals. A close RAPD marker was converted into a codominant cleaved amplified polymorphic sequence (CAPS) using specific PCR primers and restriction enzymes. This CAPS marker is tightly linked to Tsw (0.9 +/- 0.6 cM) and is helpful for marker-assisted selection in a wide range of genetic intercrosses.
辣椒对番茄斑萎病毒（TSWV）的过敏抗性由几个中国辣椒基因型中的一个显性单基因（抗性等位基因：Tsw）决定。为便于对这种抗性进行选择，利用混合分组分析法和153个F2个体，在250个10碱基对引物中筛选出4个与Tsw基因座连锁的随机扩增多态性DNA（RAPD）标记。使用特异性PCR引物和限制性内切酶将一个紧密连锁的RAPD标记转化为共显性的酶切扩增多态性序列（CAPS）标记。该CAPS标记与Tsw紧密连锁（0.9±0.6厘摩），有助于在广泛的遗传杂交中进行标记辅助选择。
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