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利用新一代测序技术开发与辣椒基因紧密连锁的分子标记

Development of molecular markers tightly linked to gene in pepper using next-generation sequencing.

作者信息

Devran Zübeyir, Kahveci Erdem, Özkaynak Ercan, Studholme David J, Tör Mahmut

机构信息

Department of Plant Protection, Faculty of Agriculture, University of Akdeniz, Antalya, Turkey.

M.Y. Genetic Agriculture Technology Laboratory, Antalya, Turkey.

出版信息

Mol Breed. 2015;35(4):101. doi: 10.1007/s11032-015-0294-5. Epub 2015 Mar 17.

DOI:10.1007/s11032-015-0294-5
PMID:25798050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4361654/
Abstract

It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. () locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked to . Initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate PCR-based markers and map . Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding site-leucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable for large-scale molecular breeding programmes.

摘要

为了进行分子标记辅助选择，识别已知性状的高度多态且紧密连锁的标记至关重要。辣椒中的（）位点赋予对马铃薯Y病毒的三种致病型以及辣椒斑驳病毒的抗性。我们描述了使用下一代测序技术来生成与该位点紧密连锁的分子标记。最初，进行了比较基因组学研究，并利用番茄10号染色体上的一个同线区域来生成基于PCR的标记并对该位点进行定位。随后，使用了辣椒的基因组序列，在该区间内鉴定出了5000多个单核苷酸变异（SNV）。此外，我们在该区间内鉴定出了核苷酸结合位点富含亮氨酸重复型抗病基因。其中几个SNV被转化为大规模分子育种计划所需的分子标记。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba62/4361654/f0bb298be545/11032_2015_294_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba62/4361654/2d2b4e99ce70/11032_2015_294_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba62/4361654/4908beefa516/11032_2015_294_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba62/4361654/f0bb298be545/11032_2015_294_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba62/4361654/2d2b4e99ce70/11032_2015_294_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba62/4361654/4908beefa516/11032_2015_294_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba62/4361654/f0bb298be545/11032_2015_294_Fig3_HTML.jpg

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