基于两种特异性抗原ESAT-6和CFP10的结核病诊断。
Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10.
作者信息
van Pinxteren L A, Ravn P, Agger E M, Pollock J, Andersen P
机构信息
Department of TB-Immunology, Statens Serum Institut, Copenhagen, Denmark.
出版信息
Clin Diagn Lab Immunol. 2000 Mar;7(2):155-60. doi: 10.1128/CDLI.7.2.155-160.2000.
Abstract
Tests based on tuberculin purified protein derivative (PPD) cannot distinguish between tuberculosis infection, Mycobacterium bovis BCG vaccination, or exposure to environmental mycobacteria. The present study investigated the diagnostic potential of two Mycobacterium tuberculosis-specific antigens (ESAT-6 and CFP10) in experimental animals as well as during natural infection in humans and cattle. Both antigens were frequently recognized in vivo and in vitro based on the induction of delayed-type hypersensitivity responses and the ability to induce gamma interferon production by lymphocytes, respectively. The combination of ESAT-6 and CFP10 was found to be highly sensitive and specific for both in vivo and in vitro diagnosis. In humans, the combination had a high sensitivity (73%) and a much higher specificity (93%) than PPD (7%).
摘要
基于结核菌素纯蛋白衍生物（PPD）的检测无法区分结核感染、卡介苗接种或环境分枝杆菌暴露。本研究调查了两种结核分枝杆菌特异性抗原（ESAT-6和CFP10）在实验动物以及人类和牛自然感染期间的诊断潜力。基于迟发型超敏反应的诱导以及淋巴细胞诱导γ干扰素产生的能力，这两种抗原在体内和体外均常被识别。发现ESAT-6和CFP10的组合对体内和体外诊断均具有高度敏感性和特异性。在人类中，该组合具有高敏感性（73%），且特异性（93%）远高于PPD（7%）。
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本文引用的文献
1
Comparative evaluation of low-molecular-mass proteins from Mycobacterium tuberculosis identifies members of the ESAT-6 family as immunodominant T-cell antigens.
Infect Immun. 2000 Jan;68(1):214-20. doi: 10.1128/IAI.68.1.214-220.2000.
2
Cellular immune responses to ESAT-6 discriminate between patients with pulmonary disease due to Mycobacterium avium complex and those with pulmonary disease due to Mycobacterium tuberculosis.
Clin Diagn Lab Immunol. 1999 Jul;6(4):606-9. doi: 10.1128/CDLI.6.4.606-609.1999.
3
Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis.
J Infect Dis. 1999 Mar;179(3):637-45. doi: 10.1086/314640.
4
Differentiation between Mycobacterium bovis BCG-vaccinated and M. bovis-infected cattle by using recombinant mycobacterial antigens.
Clin Diagn Lab Immunol. 1999 Jan;6(1):1-5. doi: 10.1128/CDLI.6.1.1-5.1999.
5
Differential T cell responses to Mycobacterium tuberculosis ESAT6 in tuberculosis patients and healthy donors.
Eur J Immunol. 1998 Dec;28(12):3949-58. doi: 10.1002/(SICI)1521-4141(199812)28:12<3949::AID-IMMU3949>3.0.CO;2-4.
6
A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10).
Microbiology (Reading). 1998 Nov;144 ( Pt 11):3195-3203. doi: 10.1099/00221287-144-11-3195.
7
Comparison of antigen-specific T-cell responses of tuberculosis patients using complex or single antigens of Mycobacterium tuberculosis.
Scand J Immunol. 1998 Nov;48(5):535-43. doi: 10.1046/j.1365-3083.1998.00419.x.
8
Use of Mycobacterium tuberculosis complex-specific antigen cocktails for a skin test specific for tuberculosis.
Infect Immun. 1998 Aug;66(8):3606-10. doi: 10.1128/IAI.66.8.3606-3610.1998.
9
MPB64 mycobacterial antigen: a new skin-test reagent through patch method for rapid diagnosis of active tuberculosis.
Int J Tuberc Lung Dis. 1998 Jul;2(7):541-6.
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Delayed-type hypersensitivity responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the guinea pig.
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