豚鼠对结核分枝杆菌ESAT-6和MPT64的迟发型超敏反应。
Delayed-type hypersensitivity responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the guinea pig.
作者信息
Elhay M J, Oettinger T, Andersen P
机构信息
Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark.
出版信息
Infect Immun. 1998 Jul;66(7):3454-6. doi: 10.1128/IAI.66.7.3454-3456.1998.
Abstract
Two antigens from Mycobacterium tuberculosis, ESAT-6 and MPT64, elicited delayed-type hypersensitivity (DTH) skin responses in outbred guinea pigs infected with M. tuberculosis by the aerosol and intravenous routes but not those sensitized with M. bovis BCG or M. avium. The DTH epitope of ESAT-6 was mapped to the C terminus. Nonresponders to the individual antigens were found, but all animals responded to a combination of ESAT-6 and MPT64 or their respective minimal target peptides. Correspondingly, these molecules could form the basis of a new skin test for tuberculosis.
摘要
结核分枝杆菌的两种抗原ESAT-6和MPT64，在通过气溶胶和静脉途径感染结核分枝杆菌的远交豚鼠中引发了迟发型超敏反应（DTH）皮肤反应，但在用牛分枝杆菌卡介苗或鸟分枝杆菌致敏的豚鼠中未引发该反应。ESAT-6的DTH表位定位于C末端。发现了对单个抗原无反应的动物，但所有动物对ESAT-6和MPT64的组合或它们各自的最小靶肽有反应。相应地，这些分子可构成一种新型结核皮肤试验的基础。
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Scand J Immunol. 1998 Nov;48(5):535-43. doi: 10.1046/j.1365-3083.1998.00419.x.
2
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3
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6
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