Sanyal P K
Biotechnology Laboratory, National Dairy Development Board, Gujarat, India.
Vet Res Commun. 2000 Feb;24(1):55-62. doi: 10.1023/a:1006377422078.
Four isolates of predacious fungi, two each of Arthrobotrys oligospora isolated from a sheep and a male crossbred calf and of Duddingtonia flagrans isolated from a sheep and a female buffalo in western India, were studied for their suitability as biocontrol agents against parasitic nematodes of ruminants, using growth assay, predatory activity, germination potential and ability to survive passing through the ruminants gut as criteria. The study showed that isolates of D. flagrans grew well in artificial media, had encouraging predatory activity, produced profuse chlamydospores that germinated easily at 25 degrees C and could survive passage through the ruminant gut. The ovine isolate of D. flagrans was superior in all respects to the isolate from buffalo and was the most promising candidate for biological control of nematode parasites of ruminants.
研究了4株捕食性真菌,其中2株少孢节丛孢分别从一只绵羊和一头雄性杂交犊牛中分离得到,另外2株弗氏新接霉分别从印度西部的一只绵羊和一头雌性水牛中分离得到,以生长测定、捕食活性、萌发潜力以及通过反刍动物肠道后的存活能力为标准,评估它们作为反刍动物寄生线虫生物防治剂的适用性。研究表明,弗氏新接霉分离株在人工培养基中生长良好,具有可观的捕食活性,产生大量在25摄氏度时易于萌发的厚垣孢子,并且能够在通过反刍动物肠道后存活。弗氏新接霉的绵羊分离株在各方面均优于水牛分离株,是反刍动物线虫寄生虫生物防治最有前景的候选菌株。