Magnarin M, Spessotto P, Soranzo M R, Pontillo A, Zabucchi G
Dipartimento di Fisiologia e Patologia, Università di Trieste, Italy.
Inflammation. 2000 Feb;24(1):89-98. doi: 10.1023/a:1006992126707.
Neutrophils and macrophages express on their membrane molecules which may, in principle, interact with each other, promote specific cell to cell adhesion, affect cell function and finally, as a consequence, modulate the progression of the inflammatory process. We tested therefore if human neutrophils specifically adhere to human monocyte-derived macrophage monolayer (MDMM). Our findings show that neutrophils significantly adhere to 4-day old MDMM and that the extent of adhesion is increased by LPS-activation of MDMM. The specificity of the interaction was shown by the very low extent of adhesion of neutrophils either to freshly prepared monocyte or other types of cell monolayers and by the low percent of adhesion showed by eosinophils exposed to 7-day old MDMM. A role for beta2 integrins, CD31 and PAF-receptor in the mechanism of neutrophil-MDMM interaction is suggested by specific antagonists. We suggest that the adhesion between the two cell types could lead to an increase in concentration of neutrophil- or macrophage released factors in the interaction site and in a mutual modulation of phagocyte functions.
中性粒细胞和巨噬细胞在其细胞膜上表达一些分子，原则上这些分子可能会相互作用，促进特定的细胞间黏附，影响细胞功能，最终进而调节炎症过程的进展。因此，我们测试了人类中性粒细胞是否能特异性黏附于人类单核细胞衍生的巨噬细胞单层（MDMM）。我们的研究结果表明，中性粒细胞能显著黏附于4天龄的MDMM，并且MDMM经脂多糖（LPS）激活后黏附程度会增加。中性粒细胞与新鲜制备的单核细胞或其他类型细胞单层的黏附程度非常低，以及嗜酸性粒细胞与7天龄MDMM接触后的低黏附百分比，均表明了这种相互作用的特异性。特异性拮抗剂表明β2整合素、CD31和血小板活化因子（PAF）受体在中性粒细胞与MDMM相互作用机制中发挥作用。我们认为，这两种细胞类型之间的黏附可能会导致相互作用部位中性粒细胞或巨噬细胞释放因子的浓度增加，并相互调节吞噬细胞功能。
