Finta C, Zaphiropoulos P G
Department of Biosciences at NOVUM, Karolinska Institute, Huddinge, SE-141 57, Sweden.
Genomics. 2000 Feb 1;63(3):433-8. doi: 10.1006/geno.1999.6063.
In human there are four known CYP2C genes that have been mapped to chromosome 10q24 with the order Cen-2C18-2C19-2C9-2C8-Tel. Previously we have shown that splicing events joining exons from the neighboring 2C18 and 2C19 genes occur in human liver and epidermis. Here evidence is presented that the terminal genes of this cluster, 2C18 and 2C8, are also involved in intergenic splicing. Most interestingly, several of these 2C18/2C8 RNAs were composed of all nine exons, thus conceivably having the potential for coding functional proteins. Moreover, chimeric RNA species consisting of exons originating not only from the CYP2C8 and CYP2C18 genes, but also from the CYP2C19 gene were detected. In all cases the exons from the different CYP2C genes were joined at the correct canonical splice sites. However, the closely linked RBP4 gene is not participating in intergenic splicing with the CYP2C genes. In addition, CYP2C8 gene expression was found to generate a variety of scrambled RNA molecules including species that contained repetitions of certain exons.
在人类中，已知有四个CYP2C基因，它们已被定位到10号染色体的10q24区域，顺序为着丝粒-2C18-2C19-2C9-2C8-端粒。此前我们已经表明，连接相邻的2C18和2C19基因外显子的剪接事件发生在人类肝脏和表皮中。本文提供的证据表明，该基因簇的末端基因2C18和2C8也参与基因间剪接。最有趣的是，这些2C18/2C8 RNA中的几种由所有九个外显子组成，因此可以想象具有编码功能蛋白的潜力。此外，还检测到嵌合RNA种类，其外显子不仅来自CYP2C8和CYP2C18基因，还来自CYP2C19基因。在所有情况下，来自不同CYP2C基因的外显子都在正确的经典剪接位点处连接。然而，紧密连锁的RBP4基因不参与与CYP2C基因的基因间剪接。此外，发现CYP2C8基因表达产生多种混乱的RNA分子，包括含有某些外显子重复的种类。
