Inoue K, Inazawa J, Suzuki Y, Shimada T, Yamazaki H, Guengerich F P, Abe T
Osaka Prefectural Institute of Public Health, Japan.
Jpn J Hum Genet. 1994 Sep;39(3):337-43. doi: 10.1007/BF01874052.
Chromosomal localization of three human cytochrome P450 genes belonging to the CYP2C subfamily (CYP2C8, 2C9, and 2C10) was identified by fluorescence in situ hybridization (FISH). An original MP-8 clone was used as a DNA probe for the assignment of the CYP2C10 gene, while two cDNA probes, a 1.37 kb fragment of CYP2C8 and a 1.19 kb fragment of CYP2C9, were obtained after amplifying the predicted fragments (MP-20 and MP-4 clones, respectively) by polymerase chain reaction using a single human liver cDNA library. The results showed that three human CYP2C8, 2C9, and 2C10 cDNAs were located at the same subchromosomal region, 10q24.1.
通过荧光原位杂交（FISH）确定了属于CYP2C亚家族的三个人细胞色素P450基因（CYP2C8、2C9和2C10）的染色体定位。一个原始的MP - 8克隆被用作CYP2C10基因定位的DNA探针，而两个cDNA探针，即CYP2C8的1.37 kb片段和CYP2C9的1.19 kb片段，是在使用单个人肝脏cDNA文库通过聚合酶链反应扩增预测片段（分别为MP - 20和MP - 4克隆）后获得的。结果表明，三个人CYP2C8、2C9和2C10的cDNA位于同一亚染色体区域10q24.1。
