对位于10q24.1的三种人类细胞色素P450 2C基因（CYP2C8、2C9和2C10）进行染色体定位的荧光原位杂交分析。

Fluorescence in situ hybridization analysis of chromosomal localization of three human cytochrome P450 2C genes (CYP2C8, 2C9, and 2C10) at 10q24.1.

作者信息

Inoue K, Inazawa J, Suzuki Y, Shimada T, Yamazaki H, Guengerich F P, Abe T

机构信息

Osaka Prefectural Institute of Public Health, Japan.

出版信息

Jpn J Hum Genet. 1994 Sep;39(3):337-43. doi: 10.1007/BF01874052.

DOI:10.1007/BF01874052

PMID:7841444

Abstract

Chromosomal localization of three human cytochrome P450 genes belonging to the CYP2C subfamily (CYP2C8, 2C9, and 2C10) was identified by fluorescence in situ hybridization (FISH). An original MP-8 clone was used as a DNA probe for the assignment of the CYP2C10 gene, while two cDNA probes, a 1.37 kb fragment of CYP2C8 and a 1.19 kb fragment of CYP2C9, were obtained after amplifying the predicted fragments (MP-20 and MP-4 clones, respectively) by polymerase chain reaction using a single human liver cDNA library. The results showed that three human CYP2C8, 2C9, and 2C10 cDNAs were located at the same subchromosomal region, 10q24.1.

摘要

通过荧光原位杂交（FISH）确定了属于CYP2C亚家族的三个人细胞色素P450基因（CYP2C8、2C9和2C10）的染色体定位。一个原始的MP - 8克隆被用作CYP2C10基因定位的DNA探针，而两个cDNA探针，即CYP2C8的1.37 kb片段和CYP2C9的1.19 kb片段，是在使用单个人肝脏cDNA文库通过聚合酶链反应扩增预测片段（分别为MP - 20和MP - 4克隆）后获得的。结果表明，三个人CYP2C8、2C9和2C10的cDNA位于同一亚染色体区域10q24.1。