Gray I C, Nobile C, Muresu R, Ford S, Spurr N K
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, England.
Genomics. 1995 Jul 20;28(2):328-32. doi: 10.1006/geno.1995.1149.
The CYP2C gene cluster on chromosome 10q24 encodes the P450IIC enzymes, members of the cytochrome P450 monooxygenase superfamily. The P450-IIC enzymes are required for the metabolism of a number of foreign compounds, including the drugs mephenytoin and tolbutamide, and are also thought to be involved in the metabolism of endogenous steroid hormones. Several different CYP2C cDNA clones have been isolated; however, the exact number of genes and the genomic arrangement of the CYP2C cluster have remained unknown. Using a combination of STS and restriction mapping to characterize YAC clones, we have constructed a 2.4-Mb physical map that incorporates the CYP2C gene cluster. The cluster spans approximately 500 kb on proximal 10q24 and comprises four genes arranged in the order CYP2C8-CYP2C9-CYP2C19-CYP2C18. The map also includes an adjacent gene, the serum retinol binding protein gene (RBP4). The incorporation of Généthon CA repeat genetic markers suggests the orientation of the loci to be Cen-RBP4-CYP2C18-CYP2C19-CYP2C9-CYP2C8-Tel .
位于10q24染色体上的CYP2C基因簇编码P450IIC酶，它是细胞色素P450单加氧酶超家族的成员。P450 - IIC酶参与多种外来化合物的代谢，包括药物美芬妥英和甲苯磺丁脲，并且也被认为参与内源性甾体激素的代谢。已经分离出几个不同的CYP2C cDNA克隆；然而，CYP2C基因簇的确切基因数量和基因组排列仍不清楚。我们使用序列标签位点（STS）和限制性图谱相结合的方法来鉴定酵母人工染色体（YAC）克隆，构建了一个包含CYP2C基因簇的2.4兆碱基物理图谱。该基因簇位于10q24近端，跨度约500千碱基，由四个基因组成，排列顺序为CYP2C8 - CYP2C9 - CYP2C19 - CYP2C18。该图谱还包括一个相邻基因，即血清视黄醇结合蛋白基因（RBP4）。整合Généthon CA重复遗传标记表明这些基因座的方向为着丝粒 - RBP4 - CYP2C18 - CYP2C19 - CYP2C9 - CYP2C8 - 端粒 。
