Zars T, Wolf R, Davis R, Heisenberg M
Theodor Boveri Institut fuer Biowissenschaften, Lehrstuhl fuer Genetik, (Biozentrum) Am Hubland, D97074, Wuerzburg, Germany.
Learn Mem. 2000 Jan;7(1):18-31. doi: 10.1101/lm.7.1.18.
Most attempts to localize physical correlates of memory in the central nervous system (CNS) rely on ablation techniques. This approach has the limitation of defining just one of an unknown number of structures necessary for memory formation. We have used the Drosophila rutabaga type I Ca(2+)/CaM-dependent adenylyl cyclase (AC) gene to determine in which CNS region AC expression is sufficient for memory formation. Using pan-neural and restricted CNS expression with the GAL4 binary transcription activation system, we have rescued the memory defect of the rutabaga mutant in a fast robust spatial learning paradigm. The ventral ganglion, antennal lobes, and median bundle are likely the CNS structures sufficient for rutabaga AC- dependent spatial learning.
大多数将记忆的物理关联定位到中枢神经系统（CNS）的尝试都依赖于切除技术。这种方法的局限性在于，它只能确定记忆形成所需的众多未知结构中的一个。我们利用果蝇rutabaga I型钙/钙调蛋白依赖性腺苷酸环化酶（AC）基因来确定在中枢神经系统的哪个区域AC表达足以形成记忆。通过GAL4二元转录激活系统进行泛神经和受限的中枢神经系统表达，我们在快速稳健的空间学习范式中挽救了rutabaga突变体的记忆缺陷。腹神经节、触角叶和中束可能是足以支持依赖rutabaga AC的空间学习的中枢神经系统结构。
