Han P L, Levin L R, Reed R R, Davis R L
Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030.
Neuron. 1992 Oct;9(4):619-27. doi: 10.1016/0896-6273(92)90026-a.
Seven lines were isolated with P element insertions in the cytogenetic vicinity of the learning and memory gene, rutabaga, from an enhancer detector screen designed to mark genes preferentially expressed in mushroom bodies. Six of these lines performed poorly in learning and memory tests, and several failed to complement an existing rutabaga allele. Molecular cloning revealed that the P elements were inserted in the putative promoter of the rutabaga gene. RNA in situ hybridization and immunohistochemistry demonstrated that the expression of the rutabaga gene, which encodes a Ca2+/calmodulin-responsive adenylyl cyclase, is markedly elevated in the mushroom bodies of normal flies and that the insertion elements compromised its expression in the new rutabaga mutants. The reisolation of a known learning and memory gene, but with a heretofore unknown expression pattern, strongly supports the postulate that mushroom bodies are principal sites mediating olfactory learning and memory.
在一项旨在标记在蘑菇体中优先表达的基因的增强子检测筛选中，从学习与记忆基因rutabaga的细胞遗传学附近区域分离出了7个带有P因子插入的品系。其中6个品系在学习和记忆测试中表现不佳，有几个品系无法与现有的rutabaga等位基因互补。分子克隆显示，P因子插入到了rutabaga基因的推定启动子中。RNA原位杂交和免疫组织化学表明，编码一种Ca2+/钙调蛋白反应性腺苷酸环化酶的rutabaga基因在正常果蝇的蘑菇体中表达显著升高，并且插入元件在新的rutabaga突变体中损害了其表达。重新分离出一个已知的学习和记忆基因，但具有迄今未知的表达模式，这有力地支持了蘑菇体是介导嗅觉学习和记忆的主要位点这一假设。
