Joiner M A, Griffith L C
Department of Biology, and Volen Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02454-9110, USA.
Learn Mem. 2000 Jan;7(1):32-42. doi: 10.1101/lm.7.1.32.
Courtship and courtship conditioning are behaviors that are regulated by multiple sensory inputs, including chemosensation and vision. Globally inhibiting CaMKII activity in Drosophila disrupts courtship plasticity while leaving visual and chemosensory perception intact. Light has been shown to modulate CaMKII-dependent memory formation in this paradigm and the circuitry for the nonvisual version of this behavior has been investigated. In this paradigm, volatile and tactile pheromones provide the primary driving force for courtship, and memory formation is dependent upon intact mushroom bodies and parts of the central complex. In the present study, we use the GAL4/UAS binary expression system to define areas of the brain that require CaMKII for modulation of courtship conditioning in the presence of visual, as well as chemosensory, information. Visual input suppressed the ability of mushroom body- and central complex-specific CaMKII inhibition to disrupt memory formation, indicating that the cellular circuitry underlying this behavior can be remodeled by changing the driving sensory modality. These findings suggest that the potential for plasticity in courtship behavior is distributed among multiple biochemically and anatomically distinct cellular circuits.
求偶行为和求偶条件反射是受多种感觉输入调节的行为，包括化学感受和视觉。在果蝇中全局抑制CaMKII活性会破坏求偶可塑性，同时保持视觉和化学感受完好无损。在这个范式中，光已被证明能调节依赖CaMKII的记忆形成，并且已经研究了这种行为的非视觉版本的神经回路。在这个范式中，挥发性和触觉性信息素为求偶提供主要驱动力，记忆形成依赖于完整的蘑菇体和中央复合体的部分区域。在本研究中，我们使用GAL4/UAS二元表达系统来确定在存在视觉以及化学感受信息的情况下，大脑中调节求偶条件反射需要CaMKII的区域。视觉输入抑制了蘑菇体和中央复合体特异性CaMKII抑制破坏记忆形成的能力，这表明这种行为的细胞神经回路可以通过改变驱动感觉模式来重塑。这些发现表明，求偶行为可塑性的潜力分布在多个生物化学和解剖学上不同的细胞回路中。
