使用氯胺-T对单克隆抗体进行高产率直接76Br-溴化反应。

High yield direct 76Br-bromination of monoclonal antibodies using chloramine-T.

作者信息

Sundin J, Tolmachev V, Koziorowski J, Carlsson J, Lundqvist H, Welt S, Larson S, Sundin A

机构信息

Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Sweden.

出版信息

Nucl Med Biol. 1999 Nov;26(8):923-9. doi: 10.1016/s0969-8051(99)00076-1.

DOI:10.1016/s0969-8051(99)00076-1

PMID:10708306

Abstract

Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean +/- max error) of 77 +/- 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean +/- max error) of 75 +/- 3%, 63 +/- 4%, and 73 +/- 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.

摘要

单克隆抗体（MAb）A33用正电子发射体76Br（半衰期T(1/2) = 16.2小时）进行标记。采用传统的氯胺-T法进行直接标记。在优化标记条件后，于pH 6.8时获得了77±2%的最大产率（平均值±最大误差）。76Br-A33与SW1222结肠癌细胞的体外结合显示其免疫反应性得以保留。此外，单克隆抗体38S1和3S193以及肽hEGF也用76Br进行了标记，标记产率（平均值±最大误差）分别为75±3%、63±4%和73±0.1%。我们得出结论，抗体和肽可用76Br方便地进行标记，用于正电子发射断层扫描全身肿瘤成像。