Niehues T, Horneff G, Knipp S, Adams O, Wahn V
Department of Pediatrics, Heinrich Heine University, Düsseldorf, Germany.
Pediatr Res. 2000 Mar;47(3):418-21. doi: 10.1203/00006450-200003000-00022.
There is a disease stage-dependent loss of CD28 expression on T cells in HIV-infected children. In this study, T cell recovery, in particular CD28 expression on T cells, was analyzed after initiation of highly active antiretroviral therapy in a group of eight mostly treatment-naive HIV-infected children. Plasma HIV-RNA levels were recorded, and numbers of CD4, CD8, CD4+CD28+, and CD8+CD28+ cells were determined by two-color flow cytometry. Values after 12 mo of therapy were compared with age-matched, seronegative control subjects. CD4 recovery to subnormal values was observed in all children. CD8+CD28+ cells recovered and were within the normal range after 12 mo of therapy (patients, 703 +/- 250 cells/microL; controls, 789 +/- 269 cells/microL), whereas CD8+CD28- cells (546 +/- 269 cells/microL) remained significantly expanded compared with age-matched controls (140 +/- 35 cells/microL). Expansions of CD8+CD28- cells persisted even in cases with long-term suppression of viral replication. Highly active antiretroviral therapy in HIV-infected children induces substantial but incomplete T cell recovery.
在感染HIV的儿童中，T细胞上CD28表达的丧失存在疾病阶段依赖性。在本研究中，对一组8名大多未接受过治疗的感染HIV的儿童启动高效抗逆转录病毒治疗后，分析了T细胞的恢复情况，尤其是T细胞上CD28的表达。记录血浆HIV-RNA水平，并通过双色流式细胞术测定CD4、CD8、CD4+CD28+和CD8+CD28+细胞的数量。将治疗12个月后的数值与年龄匹配的血清阴性对照受试者进行比较。所有儿童均观察到CD4恢复至低于正常的值。治疗12个月后，CD8+CD28+细胞恢复且在正常范围内（患者，703±250个细胞/微升；对照，789±269个细胞/微升），而CD8+CD28-细胞（546±269个细胞/微升）与年龄匹配的对照（140±35个细胞/微升）相比仍显著扩增。即使在病毒复制长期受到抑制的情况下，CD8+CD28-细胞的扩增仍持续存在。感染HIV的儿童接受高效抗逆转录病毒治疗可诱导T细胞大量但不完全恢复。
