Glaser C, Marti U, Bürgi-Saville M E, Ruchti C, Gebauer M, Büchler M W, Gerber H, Bürgi U, Peter H J
Department of Visceral Surgery, University Hospital, Bern, Switzerland.
Endocrine. 1999 Oct;11(2):165-70. doi: 10.1385/ENDO:11:2:165.
The factors mediating the accumulation of thyroglobulin are of great importance to the understanding of the pathogenesis of human and experimentally induced colloid goiters. To elucidate further the underlying cellular mechanism, thyroid fragments from newborn rats were incorporated into semisolid alginate beads and were cultured as three-dimensional organoids for up to 21 d. In five parallel cultures, the medium contained either no supplements (group A), Nal (group B), thyroid-stimulating hormone (TSH) (group C), Nal plus TSH in the same concentrations as B and C (group D), or Nal and TSH (as in group D) plus methimazole (MMI, group E). The thyroid organoids maintained morphological integrity, functional activity, and ability to proliferate in vitro. Addition of iodine to the cultures significantly increased mean (+/-SEM) follicular diameters from 19.5 +/- 0.7 microm in controls to 33.9 +/- 2.2 microm (p < 0.0001) when NaI was added alone (group B), and 30.4 +/- 1.7 microm (p < 0.0001) when combined with TSH (group D). The effect of NaI on follicular size was abolished by MMI (group E, follicular diameter 23.5 +/- 1.3 microm). The results presented support the recent finding, using a rat colloid goiter model, that not only TSH but also iodine organification or its inhibition are important factors in modulating follicular morphology.
介导甲状腺球蛋白积聚的因素对于理解人类和实验性诱导的胶样甲状腺肿的发病机制非常重要。为了进一步阐明潜在的细胞机制，将新生大鼠的甲状腺碎片包埋在半固体藻酸盐珠中，并作为三维类器官培养长达21天。在五个平行培养物中，培养基要么不添加任何补充剂（A组），添加碘化钠（B组），添加促甲状腺激素（TSH）（C组），添加与B组和C组相同浓度的碘化钠加TSH（D组），要么添加碘化钠和TSH（如D组）再加甲巯咪唑（MMI，E组）。甲状腺类器官在体外保持形态完整性、功能活性和增殖能力。当单独添加碘化钠时（B组），向培养物中添加碘可使平均（±SEM）滤泡直径从对照组的19.5±0.7微米显著增加至33.9±2.2微米（p<0.0001），当与TSH联合添加时（D组）增加至30.4±1.7微米（p<0.0001）。甲巯咪唑消除了碘化钠对滤泡大小的影响（E组，滤泡直径23.5±1.3微米）。所呈现的结果支持了最近使用大鼠胶样甲状腺肿模型的研究发现，即不仅TSH，而且碘的有机化或其抑制都是调节滤泡形态的重要因素。
