Patey M, Delemer B, Bellon G, Martiny L, Pluot M, Haye B
Service d'Anatomie et de Cytologie Pathologiques, CHU Robert Debré, Université Reims Champagne Ardenne, France.
J Clin Pathol. 1999 Dec;52(12):895-900. doi: 10.1136/jcp.52.12.895.
To describe the pattern of distribution of thrombospondin (TSP1) and its receptors, alpha root of beta 3 integrin and CD36, in normal human thyroid tissue and to compare their expression in different benign and malignant thyroid conditions.
Immunohistochemistry was used to study TSP1 and its receptors in 40 surgical thyroidectomy specimens (normal parenchyma, 7; follicular adenoma, 4; multinodular goitre, 13; papillary carcinoma, 6; follicular carcinoma, 8; anaplastic carcinoma, 2).
In the normal thyroid parenchyma, there was weak expression of TSP1 limited to the vessels with no staining of the extracellular matrix. In goitres, the expression of TSP1 was more pronounced in areas of fibrosis, with staining of alpha root of beta 3 on thyrocytes located in the vicinity. In thyroid adenomas, expression of TSP1 was slightly enhanced compared with normal tissue, located in the basement membrane of vessels. In papillary carcinomas, TSP1 was abundant in the desmoplastic stroma with a cytoplasmic distribution of alpha root of beta 3 integrin in thyrocytes. In follicular carcinomas, TSP1 was less abundant in the extracellular matrix, limited to the vessels of the stroma with a weaker expression of alpha root of beta 3 on thyrocytes than in papillary carcinomas. In anaplastic carcinomas, TSP1 was only present in the numerous capillaries of the stroma, with a marked positivity for alpha root of beta 3 in one case. No immunostaining of thyrocytes is observed with CD36.
These results suggest the importance of the interaction between alpha root of beta 3 integrin and TSP1 during remodelling of the matrix in fibrous goitres with areas of early sclerosis comparable with wound healing. In papillary carcinomas, the overexpression of TSP1 restricted to the stroma suggests protective effects against tumour progression.
描述血小板反应蛋白-1（TSP1）及其受体β3整合素α亚基和CD36在正常人类甲状腺组织中的分布模式，并比较它们在不同良性和恶性甲状腺疾病中的表达情况。
采用免疫组织化学方法研究40例手术切除的甲状腺标本（正常实质组织7例、滤泡性腺瘤4例、结节性甲状腺肿13例、乳头状癌6例、滤泡状癌8例、未分化癌2例）中TSP1及其受体的表达。
在正常甲状腺实质组织中，TSP1呈弱表达，仅限于血管，细胞外基质无染色。在甲状腺肿中，TSP1在纤维化区域表达更明显，邻近的甲状腺细胞上有β3整合素α亚基染色。在甲状腺腺瘤中，TSP1的表达与正常组织相比略有增强，位于血管基底膜。在乳头状癌中，TSP1在促纤维增生性基质中丰富，甲状腺细胞中β3整合素α亚基呈胞质分布。在滤泡状癌中，细胞外基质中TSP1较少，仅限于基质血管，甲状腺细胞上β3整合素α亚基的表达比乳头状癌弱。在未分化癌中，TSP1仅存在于基质的众多毛细血管中，1例β3整合素α亚基呈明显阳性。未观察到甲状腺细胞CD36免疫染色。
这些结果表明，在与伤口愈合类似的早期硬化区域的纤维性甲状腺肿基质重塑过程中，β3整合素α亚基与TSP1之间相互作用具有重要意义。在乳头状癌中，TSP1仅限于基质的过表达提示对肿瘤进展有保护作用。
