Matthews V, Robertson T, Kendrick T, Abdo M, Papadimitriou J, McMinn P
Department of Microbiology, Queen Elizabeth II Medical Centre; Department of Anatomy and Human Biology, University of Western Australia, Nedlands, Australia.
Int J Exp Pathol. 2000 Feb;81(1):31-40. doi: 10.1046/j.1365-2613.2000.00135.x.
We have examined the histological and ultrastructural features of CNS infection with Murray Valley encephalitis (MVE) virus in mice inoculated with a virulent parental strain (BH3479). Light microscopic examination revealed neuronal necrosis in the olfactory bulb and hippocampus of MVE-infected brains by 5 days post-infection (pi). Electron microscopy of these regions showed endoplasmic reticulum membrane proliferation, and tubular and spherical structures in the cisternae of the endoplasmic reticulum, Golgi complex and nuclear envelope. At seven to eight days pi, infected neurones exhibited chromatin condensation and extrusion, nuclear fragmentation, loss of segments of the nuclear envelope, reduced surface contact with adjacent cells and loss of cytoplasmic organelles. This cell injury was particularly noticeable in the proximal CA3 and distal CA1 regions of the hippocampus. The inflammatory cell profile consisted of macrophages, lymphocytes and especially neutrophils, and many of these inflammatory cells were apoptotic. High mortality rates in the BH3479-infected population of mice correlated with the intense polymorphonuclear and mononuclear leucocyte inflammatory infiltrate in the CNS.
我们研究了用强毒株亲本株（BH3479）接种的小鼠感染墨累谷脑炎（MVE）病毒后中枢神经系统感染的组织学和超微结构特征。光学显微镜检查显示，感染MVE的小鼠在感染后5天，嗅球和海马体出现神经元坏死。这些区域的电子显微镜检查显示内质网膜增殖，内质网、高尔基体复合体和核膜池中有管状和球形结构。感染后7至8天，受感染的神经元表现出染色质浓缩和挤出、核碎裂、核膜片段丢失、与相邻细胞的表面接触减少以及细胞质细胞器丢失。这种细胞损伤在海马体的近端CA3区和远端CA1区尤为明显。炎症细胞谱包括巨噬细胞、淋巴细胞，尤其是中性粒细胞，其中许多炎症细胞发生凋亡。BH3479感染的小鼠群体中的高死亡率与中枢神经系统中强烈的多形核和单核白细胞炎性浸润相关。
