Wrynn A S, Sebens J B, Koch T, Leonard B E, Korf J
Department of Pharmacology, National University of Ireland, Galway, Ireland.
Brain Res Mol Brain Res. 2000 Mar 10;76(1):7-17. doi: 10.1016/s0169-328x(99)00326-5.
Olfactory bulbectomy is a well established animal model of depression. Neurochemical and behavioral alterations observed following olfactory bulbectomy, are due, in part, to the neurodegeneration of specific brain structures. Amygdaloid dysfunction in particular, is known to play a substantial role in the syndrome of the olfactory bulbectomized rat. The present study examined both short- and long-term alterations in immediate early gene expression, tyrosine hydroxylase and serotonin immunoreactivity, and classical silver staining, following olfactory bulbectomy in the basolateral amygdala. The results indicated no consistent change in Fos expression observed over the experimental period. Following bulbectomy, long term (up to 64 days post-lesion) Jun expression, not coincident with silver staining, was observed in the basolateral nucleus. The basolateral nucleus was also intensely immunoreactive for serotonin at this timepoint post-bulbectomy. Thus, following bulbectomy long term alterations in Jun expression occurs in the serotonin rich basolateral amygdala. As a site of action for antidepressant compounds, alterations at the immediate early gene level in this region may have implications both for the model, and antidepressant drug action therein.
嗅球切除术是一种成熟的抑郁症动物模型。嗅球切除术后观察到的神经化学和行为改变，部分归因于特定脑结构的神经退行性变。特别是杏仁核功能障碍，在嗅球切除大鼠综合征中起着重要作用。本研究检测了嗅球切除术后基底外侧杏仁核中即刻早期基因表达、酪氨酸羟化酶和5-羟色胺免疫反应性以及经典银染的短期和长期变化。结果表明，在实验期间未观察到Fos表达的一致变化。嗅球切除术后，在基底外侧核观察到长期（损伤后长达64天）的Jun表达，且与银染不一致。在嗅球切除术后的这个时间点，基底外侧核的5-羟色胺免疫反应也很强。因此，嗅球切除术后，富含5-羟色胺的基底外侧杏仁核中Jun表达出现长期改变。作为抗抑郁化合物的作用部位，该区域即刻早期基因水平的改变可能对该模型以及其中的抗抑郁药物作用都有影响。
