血清素的抗氧化特性与多形核吞噬细胞的杀菌功能

Antioxidative properties of serotonin and the bactericidal function of polymorphonuclear phagocytes.

作者信息

Schuff-Werner P, Splettstoesser W

机构信息

Institute of Clinical Chemistry and Pathobiochemistry, University of Rostock, Medical Faculty, Germany.

出版信息

Adv Exp Med Biol. 1999;467:321-5. doi: 10.1007/978-1-4615-4709-9_41.

DOI:10.1007/978-1-4615-4709-9_41

PMID:10721072

Abstract

We investigated the antioxidative properties of platelet-released serotonin on the bactericidal function of polymorphonuclear neutrophils (PMN) since there is a surprising co-incidence of low blood serotonin and an increased rate of infections. The antioxidative properties of serotonin were demonstrated by its suppressive effects on phagocytosis-associated, luminol-enhanced chemiluminescence (CL). The bactericidal activity of PMN was determined by a microbiological assay using opsonized Staphylococcus aureus. Serotonin suppresses luminol-enhanced chemiluminescence in a dose-dependent manner indicating an interaction with reactive oxygen species, which are responsible for effective bacterial killing during the phagocytosis-associated "respiratory burst". The modulation of the bactericidal function of PMN by serotonin is complex and depends upon the amount of serotonin: at concentrations normally present at sites of tissue injury and consecutive thrombus formation (10(-6) to 10(-5) M) bacterial killing increases by about 50%. In contrast, at pharmacological concentrations (10(-3) to 10(-2) M) an adverse effect can be observed: the elimination of opsonized S. aureus is reduced by 30 to 90%. Exogenous serotonin is capable of modulating important biological functions of human PMN in vitro. At appropriate concentrations, the antibacterial defence improves significantly probably due to reduced autooxidation, whereas higher concentrations counteract an efficient bacterial killing.

摘要

鉴于血液中血清素水平较低与感染率上升这一惊人的巧合，我们研究了血小板释放的血清素对多形核中性粒细胞（PMN）杀菌功能的抗氧化特性。血清素的抗氧化特性通过其对吞噬作用相关的、鲁米诺增强的化学发光（CL）的抑制作用得以证明。PMN的杀菌活性通过使用调理过的金黄色葡萄球菌的微生物学检测来确定。血清素以剂量依赖性方式抑制鲁米诺增强的化学发光，表明其与活性氧发生相互作用，而活性氧在吞噬作用相关的“呼吸爆发”过程中负责有效杀灭细菌。血清素对PMN杀菌功能的调节较为复杂，且取决于血清素的量：在组织损伤部位和随后血栓形成部位通常存在的浓度（10⁻⁶至10⁻⁵ M）下，细菌杀灭率增加约50%。相比之下，在药理浓度（10⁻³至10⁻² M）下可观察到不利影响：调理过的金黄色葡萄球菌的清除率降低30%至90%。外源性血清素能够在体外调节人类PMN的重要生物学功能。在适当浓度下，抗菌防御可能由于自身氧化减少而显著改善，而较高浓度则会对抗有效的细菌杀灭。