Johansen K S, Berger E M, Repine J E
Acta Pathol Microbiol Immunol Scand C. 1983 Dec;91(6):355-9.
The effect of temperature on the function of polymorphonuclear leukocytes (PMN) has been investigated in vitro. Increases in temperature from 37 degrees C to 40 degrees C progressively increased chemiluminescence (CL) responses by PMN after stimulation by Staphylococcus aureus, zymosan or phorbol myristate acetate (PMA) while increases above 40 degrees C decreased these functions. Temperature increases from 37 degrees C to 40 degrees C also produced increased PMN bactericidal activity against S. aureus. In contrast, similar increases in temperature did not change superoxide production by PMN stimulated by PMA. Incubation of PMN at the various temperatures did not cause release of LDH indicating that damage to PMN was not the cause of reduced PMN chemiluminescence and bactericidal activity seen within the temperature range studied. The discrepancy between the influence of temperature on PMN chemiluminescence and bactericidal activity of PMN compared to superoxide anion production by PMN suggests that superoxide anion production may not be solely, or at least directly, responsible for killing of bacteria. Careful temperature control is needed when assaying PMN function. Febrile responses up to 40 degrees C may play a beneficial role in host defense.
已在体外研究了温度对多形核白细胞（PMN）功能的影响。温度从37℃升高到40℃时，金黄色葡萄球菌、酵母聚糖或佛波酯肉豆蔻酸酯乙酸酯（PMA）刺激后PMN的化学发光（CL）反应逐渐增强，而温度高于40℃时这些功能则下降。温度从37℃升高到40℃也使PMN对金黄色葡萄球菌的杀菌活性增强。相比之下，类似的温度升高并未改变PMA刺激的PMN产生超氧化物的情况。在不同温度下孵育PMN并未导致乳酸脱氢酶（LDH）释放，这表明在所研究的温度范围内，PMN化学发光和杀菌活性降低并非PMN受损所致。与PMN产生超氧阴离子相比，温度对PMN化学发光和杀菌活性的影响存在差异，这表明超氧阴离子的产生可能并非唯一或至少不是直接导致细菌杀灭的原因。在检测PMN功能时需要仔细控制温度。高达40℃的发热反应可能在宿主防御中发挥有益作用。
