Ivanov A R, Nazimov I V
Analytical Protein Chemistry Department, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
J Chromatogr A. 2000 Feb 18;870(1-2):255-69. doi: 10.1016/s0021-9673(99)01058-4.
The techniques for micro-level analysis of some widespread unusual amino acids (phosphorylated and hydroxylated ones) as well as of some genetically non-encoded amino acids were developed for their subsequent identification in the peptide and protein amino acid sequence by narrow-bore column high-performance liquid chromatography (approximately 10 pmol of the sample), high-performance capillary electrophoresis (approximately 1-10 pmol), matrix-assisted laser desorption ionization time-of-flight mass spectrometry (approximately 1-10 pmol), and automatic protein gas phase sequencing (approximately 1-50 pmol).
已开发出针对某些广泛存在的特殊氨基酸（磷酸化和羟基化氨基酸）以及一些基因非编码氨基酸的微观层面分析技术，以便随后通过窄孔柱高效液相色谱法（约10皮摩尔样品）、高效毛细管电泳法（约1 - 10皮摩尔）、基质辅助激光解吸电离飞行时间质谱法（约1 - 10皮摩尔）和自动蛋白质气相测序法（约1 - 50皮摩尔）对肽和蛋白质氨基酸序列中的这些氨基酸进行鉴定。
