Ersin S, Tuncyurek P, Esassolak M, Alkanat M, Buke C, Yilmaz M, Telefoncu A, Kose T
Department of Surgery, Aegean University Faculty of Medicine, Izmir, Turkey.
J Surg Res. 2000 Apr;89(2):121-5. doi: 10.1006/jsre.1999.5808.
Recent studies indicated that glutamine and arginine support the mucosal barrier in several ways. This experimental study hypothesized that administration of glutamine- and arginine-enriched diets before abdominal radiation therapy would provide a radioprotective effect on intestinal mucosa, and this would augment the therapeutic effectiveness provided by postirradiation administration.
A rat model of radiation enteritis was designed with a single dose of 1100 cGy to the abdomen. Thirty-five rats were randomized into five groups of seven. A 7-day glutamine-enriched diet for Group I and a 7-day arginine-enriched diet for Group II were administered both pre- and postradiation. For Groups III and IV, the same glutamine and arginine diets were given, respectively, postradiation only. Group V was fed a glutamine- and arginine-free diet and was the control group. The rats underwent laparotomy for culture of mesenteric lymph nodes and removal of segments of ileum, jejenum, and colon for microscopic examination.
Bacterial translocation was significantly higher in Group V (P < 0.05), while intestinal villus count and villus height were significantly higher in all of the groups fed glutamine and arginine when compared with the control group (P < 0.0001 and P < 0.05, respectively).
Both arginine- and glutamine-enriched diets have protective effects on gut mucosa in the postirradiation state; however, pre- and postirradiation administration together does not provide superior protection versus postradiation administration alone.
近期研究表明，谷氨酰胺和精氨酸可通过多种方式维持黏膜屏障。本实验研究假设，在腹部放射治疗前给予富含谷氨酰胺和精氨酸的饮食，将对肠黏膜产生辐射防护作用，且这将增强放疗后给药所提供的治疗效果。
设计腹部单次照射剂量为1100 cGy的放射性肠炎大鼠模型。35只大鼠随机分为5组，每组7只。第I组给予富含谷氨酰胺的饮食7天，第II组给予富含精氨酸的饮食7天，两组均在放疗前后给药。第III组和第IV组分别仅在放疗后给予相同的谷氨酰胺和精氨酸饮食。第V组喂食不含谷氨酰胺和精氨酸的饮食，为对照组。大鼠接受剖腹手术以培养肠系膜淋巴结，并切除回肠、空肠和结肠段进行显微镜检查。
第V组的细菌移位显著更高（P < 0.05），而与对照组相比，所有喂食谷氨酰胺和精氨酸的组的肠绒毛计数和绒毛高度均显著更高（分别为P < 0.0001和P < 0.05）。
富含精氨酸和谷氨酰胺的饮食对放疗后状态的肠道黏膜均有保护作用；然而，放疗前后联合给药与仅放疗后给药相比，并未提供更好的保护。
