Olson P E, Kallen A J, Bjorneby J M, Creek J G
Epidemiology Department, Naval Station, San Diego, CA 92136, USA.
J Vet Diagn Invest. 2000 Mar;12(2):126-9. doi: 10.1177/104063870001200204.
Prevalence of Lyme borreliosis in canine sentinels has been shown to correlate with infection in humans. One thousand canine sera (917 dogs, 83 coyotes) obtained from animal control authorities and area veterinarians were screened by ELISA for antibodies to Borrelia burgdorferi. Results were validated by Western blot and indirect fluorescent antibody (IFA) tests at referee laboratories. Criterion for a positive Western blot was presence of 5 of 10 of the most common antigen IgG bands; for IFA, >1:128 or the equivalent when correcting for interlaboratory variability. Twenty-two of 1,000 canines were confirmed serologically positive (21 dogs and 1 coyote; seroprevalence 2.3% and 1.2%, respectively). Lifestyle, breed size, gender, and age were not statistically predictive of seropositive status. No regional clustering of seropositive animals was detected. The low prevalence of seropositivity in sentinel canines suggests the Lyme borreliosis hazard in San Diego County is minimal.
犬类哨兵中莱姆病疏螺旋体病的患病率已被证明与人类感染相关。从动物控制部门和当地兽医处获取的1000份犬血清样本（917只狗，83只郊狼）通过酶联免疫吸附测定法（ELISA）筛查抗伯氏疏螺旋体抗体。结果在参比实验室通过免疫印迹法和间接荧光抗体（IFA）检测进行验证。免疫印迹法阳性标准为10条最常见抗原IgG条带中出现5条；IFA检测中，校正实验室间差异后，滴度>1:128或等效滴度。1000只犬中有22只经血清学确认呈阳性（21只狗和1只郊狼；血清阳性率分别为2.3%和1.2%）。生活方式、品种大小、性别和年龄在统计学上均不能预测血清阳性状态。未检测到血清阳性动物的区域聚集现象。哨兵犬血清阳性率较低表明圣地亚哥县的莱姆病疏螺旋体病危害极小。
