Delgado S, Cármenes P
Departamento de Sanidad Animal, Enfermedades Infecciosas y Epidemiología, Facultad de Veterinaria, Universidad de León, Spain.
Eur J Epidemiol. 1995 Jun;11(3):321-4. doi: 10.1007/BF01719437.
A random epidemiological study was undertaken to estimate the prevalence of canine borreliosis (Lyme disease) in Castilla y León, the largest region in Spain. The presence of antibodies was determined by indirect immunofluorescence assay (IFA), using the Borrelia burgdorferi B31 strain as antigen. Sera from 308 dogs from 7 provinces in the region were tested. Of all the animals sampled, 37 (21%) were seropositive (titres of 1/64 or above). Almost all the provinces had seroprevalence of 20% or below, except for an important focus of borreliosis in the Zamora province (42.8%). Potential risk factors (age, sex, use, habitat, season, and presence of ticks on the animals) relating to the presence of antibodies were evaluated. Those dogs which had at some time had ticks were more often seropositive, at 24.2%, than those which had never had them, at only 6.2% (p < 10-n). No significant differences were discovered for the remaining factors studied. This work indicates that dogs in Castilla y León, even if in most cases they do not develop the disease, are exposed to the Lyme disease agent.
开展了一项随机流行病学研究，以估算西班牙最大的地区卡斯蒂利亚-莱昂犬类莱姆病的患病率。采用间接免疫荧光测定法（IFA），以伯氏疏螺旋体B31菌株作为抗原，测定抗体的存在情况。对该地区7个省份的308只犬的血清进行了检测。在所有采样动物中，37只（21%）血清呈阳性（滴度为1/64或更高）。除了萨莫拉省存在一个重要的莱姆病疫源地（42.8%）外，几乎所有省份的血清阳性率均在20%或以下。对与抗体存在相关的潜在风险因素（年龄、性别、用途、栖息地、季节以及动物身上是否有蜱虫）进行了评估。那些曾在某个时候感染过蜱虫的犬血清呈阳性的比例更高，为24.2%，而那些从未感染过蜱虫的犬血清呈阳性的比例仅为6.2%（p < 10-n）。在研究的其余因素中未发现显著差异。这项研究表明，卡斯蒂利亚-莱昂的犬类即使在大多数情况下不会发病，但仍接触到莱姆病病原体。
