Choi C, Chae C, Clark E G
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agriculture and Biotechnology, Seoul National University, Suwon, Republic of Korea.
J Vet Diagn Invest. 2000 Mar;12(2):151-3. doi: 10.1177/104063870001200209.
This report describes the first diagnosis of porcine circovirus (PCV) infection in weaned pigs with postweaning multisystemic wasting syndrome in Korea by immunohistochemistry and polymerase chain reaction. The most unique lesions were multifocal granulomatous inflammation affecting lymph nodes, liver, and spleen, characterized by infiltrates of epithelioid macrophages and multinucleated giant cells. Circoviral antigen was detected in formalin-fixed sections and was usually present in large, round, dendritic cells in the white pulp of spleen and remnants of follicles in lymph nodes. Lymphoid follicles in the tonsils also contained PCV antigen. A 530-bp DNA fragment of circovirus was successfully amplified from all tested lymph nodes, liver, and spleen.
本报告描述了通过免疫组织化学和聚合酶链反应首次在韩国断奶仔猪中诊断出猪圆环病毒（PCV）感染伴断奶后多系统消耗综合征。最独特的病变是多灶性肉芽肿性炎症，累及淋巴结、肝脏和脾脏，其特征为上皮样巨噬细胞和多核巨细胞浸润。在福尔马林固定切片中检测到圆环病毒抗原，其通常存在于脾脏白髓中的大型圆形树突状细胞以及淋巴结滤泡残余中。扁桃体中的淋巴滤泡也含有PCV抗原。从所有检测的淋巴结、肝脏和脾脏中成功扩增出一段530bp的圆环病毒DNA片段。
